DEVICES AND PROCESSES FOR NUCLEIC ACID EXTRACTION
First Claim
Patent Images
1. A device comprising:
- (i) a body member having a plurality of external surfaces and fabricated to contain a continuous fluid pathway therethrough, the pathway comprising;
a first port;
a second port; and
a binding channel intermediate and in fluid communication with the first port and the second port, wherein the binding channel is open to one of the external surfaces of the body member; and
(ii) a glass member affixed to said one of the external surfaces of the body member to provide a first unmodified flat glass surface in fluid communication with the binding channel, wherein the binding channel and glass member define a binding chamber effective for binding a heterogeneous population of nucleic acids and wherein the fluid pathway is essentially free of nucleic acid-specific binding sites.
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Abstract
Devices, processes, and kits for the extraction of nucleic acids from biological samples are disclosed. The devices comprise a first port, a second port, and a binding chamber intermediate and in fluid communication with the first port and the second port. The binding chamber comprises an unmodified flat glass surface effective for binding a heterogeneous population of nucleic acids. The first port, second port, and binding chamber define a continuous fluid pathway that is essentially free of nucleic acid-specific binding sites.
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Citations
49 Claims
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1. A device comprising:
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(i) a body member having a plurality of external surfaces and fabricated to contain a continuous fluid pathway therethrough, the pathway comprising; a first port; a second port; and a binding channel intermediate and in fluid communication with the first port and the second port, wherein the binding channel is open to one of the external surfaces of the body member; and (ii) a glass member affixed to said one of the external surfaces of the body member to provide a first unmodified flat glass surface in fluid communication with the binding channel, wherein the binding channel and glass member define a binding chamber effective for binding a heterogeneous population of nucleic acids and wherein the fluid pathway is essentially free of nucleic acid-specific binding sites. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49)
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18. A device comprising:
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(i) a body member having a plurality of external surfaces and fabricated to contain a continuous fluid pathway therethrough, the pathway comprising; a first port; a second port; a binding channel intermediate and in fluid communication with the first port and the second port, wherein the binding channel is open to one of the external surfaces of the body member; a distribution channel in fluid communication with the binding channel; and a plurality of capillary channels in fluid communication with the distribution channel distal to the binding channel; and (ii) a glass member affixed to said one of the external surfaces of the body member to provide a first unmodified flat glass surface in fluid communication with the binding channel;
wherein the binding channel and glass member define a binding chamber effective for binding a heterogeneous population of nucleic acids. - View Dependent Claims (19)
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20. A device comprising:
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(i) a body member having a plurality of external surfaces and fabricated to contain a single continuous fluid pathway therethrough, the pathway consisting essentially of; a first channel; a second channel; a binding channel between the first channel and the second channel, wherein the binding channel is open to one of the external surfaces of the body member; a plurality of ports, wherein at least one of the ports is in fluid communication with the first channel distal to the binding channel, and wherein at least another of the ports is in fluid communication with the second channel distal to the binding channel; and (ii) a glass member affixed to said one of the external surfaces of the body member to provide a first unmodified flat glass surface in fluid communication with the binding channel, wherein the binding channel and glass member define a binding chamber effective for binding a heterogeneous population of nucleic acids and wherein the fluid pathway is essentially free of nucleic acid-specific binding sites. - View Dependent Claims (21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32)
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Specification