METHODS OF PRODUCING TRANSFORMATION COMPETENT BACTERIA
First Claim
1. A method of producing transformation competent bacteria, comprising the steps of:
- (i) transforming a bacteria that is not naturally transformation competent with a plasmid, wherein said plasmid comprises a comX gene sequence encoding a ComX protein or functional part or derivative or variant thereof under the regulatory control of a promoter which is inducible by a transcription initiator,(ii) contacting said transformed bacteria with said transcription initiator to initiate transcription of said comX gene sequence.
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Abstract
A method of producing transformation competent bacteria, comprising the steps of: (i) transforming a bacteria that is not naturally transformation competent with a plasmid, wherein said plasmid comprises a comX gene sequence encoding a ComX protein or functional part or derivative or variant thereof under the regulatory control of a promoter which is inducible by a transcription initiator, (ii) contacting said transformed bacteria with said transcription initiator to initiate transcription of said comX gene sequence is provided. Also provided are plasmids, transformed bacteria, transformation competent bacteria, mutant bacteria and food products comprising said mutant bacteria.
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Citations
42 Claims
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1. A method of producing transformation competent bacteria, comprising the steps of:
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(i) transforming a bacteria that is not naturally transformation competent with a plasmid, wherein said plasmid comprises a comX gene sequence encoding a ComX protein or functional part or derivative or variant thereof under the regulatory control of a promoter which is inducible by a transcription initiator, (ii) contacting said transformed bacteria with said transcription initiator to initiate transcription of said comX gene sequence. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 18, 19, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37)
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17. A method of producing transformation competent S. thermophilus bacteria, comprising at least the steps of:
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(i) transforming a S. thermophilus bacteria with a plasmid, wherein said plasmid comprises a comX gene sequence which comprises; (a) the nucleotide sequence of SEQ ID NO;
1;
or(b) a portion thereof;
or(c) a sequence which hybridizes to said sequence or portion thereof under non-stringent binding conditions and washing under conditions of high stringency;
or(d) a sequence which exhibits at least 80% sequence identity to said sequence or portion thereof;
or(e) a sequence complementary to any of the aforesaid sequences; under the regulatory control of a promoter comprising; (a) the nucleotide sequence of SEQ ID NO;
3;
or(b) a portion thereof;
or(c) a sequence which hybridizes to said sequence or portion thereof under non-stringent binding conditions and washing under conditions of high stringency;
or(d) a sequence which exhibits at least 80% sequence identity to said sequence or portion thereof;
or(e) a sequence complementary to any of the aforesaid sequences; which is inducible by a transcription initiator comprising; (a) the amino acid sequence of SEQ ID NO;
4;
or(b) a portion thereof;
or(c) a sequence which exhibits at least 80% sequence identity to said sequence or portion thereof, optionally further comprising a reporter gene under the control of said promoter; and (ii) contacting said transformed bacteria with said transcription initiator to initiate transcription of said comX gene sequence. - View Dependent Claims (38, 39, 40, 41, 42)
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- 20. A plasmid comprising a comX gene sequence encoding a ComX protein or functional part or derivative or variant thereof under the regulatory control of a promoter which is inducible by a transcription initiator.
Specification