BIOSENSORS AND METHODS FOR MAKING AND USING THEM
First Claim
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1. A method of immobilizing a protein on a rigid macroporous polymer comprising the steps of:
- (a) combining the protein with the rigid macroporous polymer having functional moieties capable of crosslinking to a protein; and
(b) adding a crosslinking agent capable of immobilizing the protein on the rigid macroporous polymer by crosslinking the functional moieties of the protein with the functional moieties of the rigid macroporous polymerso that the protein is immobilized on the rigid macroporous polymer.
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Abstract
Embodiments of the invention provide analyte sensors having optimized permselective membranes and methods for making and using such sensors. Embodiments of the invention also provide analyte sensors such as those having porous matrices coated with an analyte sensing composition and methods for making and using such sensors. Illustrative embodiments include electrochemical glucose sensors having glucose oxidase coatings.
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6 Claims
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1. A method of immobilizing a protein on a rigid macroporous polymer comprising the steps of:
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(a) combining the protein with the rigid macroporous polymer having functional moieties capable of crosslinking to a protein; and (b) adding a crosslinking agent capable of immobilizing the protein on the rigid macroporous polymer by crosslinking the functional moieties of the protein with the functional moieties of the rigid macroporous polymer so that the protein is immobilized on the rigid macroporous polymer. - View Dependent Claims (2, 5, 6)
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- 3. A method of immobilizing a protein on a rigid macroporous polymer comprising combining a protein having a sulfhydryl, amine, carboxyl or hydroxyl moiety with a rigid macroporous polymer having reactive epoxide moieties under reaction conditions that allow a nucleophilic reaction to occur between the sulfhydryl, amine, carboxyl or hydroxyl moieties on the protein and the epoxide moieties on the rigid macroporous polymer so that the protein is immobilized on the rigid macroporous polymer.
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