BIOSENSORS AND METHODS FOR MAKING AND USING THEM

• US 20100279377A1
• Filed: 05/20/2010
• Published: 11/04/2010
• Est. Priority Date: 12/13/2005
• Status: Abandoned Application

• Current Assignee: Medtronic Minimed Incorporated (Medtronic Plc)
• Original Assignee: Medtronic Minimed Incorporated (Medtronic Plc)
• Inventors: Shah, Rajiv, Hoss, Udo, Soundararajan, Gopikrishnan, Grovender, Eric A., Gottlieb, Rebecca K., Pendo, Shaun M.
• Application Number:
  US12/784,233
• Publication Number:
  US 20100279377A1
• Time in Patent Office: Days
• US Class Current: 435/180
• CPC Class Codes: C12Q 1/006 for glucose

Abstract

Embodiments of the invention provide analyte sensors having optimized permselective membranes and methods for making and using such sensors. Embodiments of the invention also provide analyte sensors such as those having porous matrices coated with an analyte sensing composition and methods for making and using such sensors. Illustrative embodiments include electrochemical glucose sensors having glucose oxidase coatings.

Claims

1. A method of immobilizing a protein on a rigid macroporous polymer comprising the steps of:

(a) combining the protein with the rigid macroporous polymer having functional moieties capable of crosslinking to a protein; and

(b) adding a crosslinking agent capable of immobilizing the protein on the rigid macroporous polymer by crosslinking the functional moieties of the protein with the functional moieties of the rigid macroporous polymer

so that the protein is immobilized on the rigid macroporous polymer.

2. The method of claim 1, wherein the rigid macroporous polymer having functional moieties capable of crosslinking to a protein is made by combining a rigid macroporous polymer having reactive epoxide moieties with a nucleophilic compound so that a rigid macroporous polymer having functional moieties capable of crosslinking to a protein is made.

5. The composition of claim 1, wherein the rigid macroporous polymer comprises a plurality of pores and further wherein at least one of the plurality of pores contains a biocompatible hydrophilic polymer that facilitates hydration of the composition.

6. The composition of claim 1, wherein the rigid macroporous polymer comprises a plurality of pores and further wherein at least one of the plurality of pores contains functionalized poly(dimethyl siloxane), functionalized poly(dimethyl siloxane) copolymer or a mixture of functionalized poly(dimethyl siloxane) and functionalized poly(dimethyl siloxane) copolymer of the second layer.

3. A method of immobilizing a protein on a rigid macroporous polymer comprising combining a protein having a sulfhydryl, amine, carboxyl or hydroxyl moiety with a rigid macroporous polymer having reactive epoxide moieties under reaction conditions that allow a nucleophilic reaction to occur between the sulfhydryl, amine, carboxyl or hydroxyl moieties on the protein and the epoxide moieties on the rigid macroporous polymer so that the protein is immobilized on the rigid macroporous polymer.

4. The method of claim 3, wherein at least one nucleophilic moiety on the protein is blocked prior to combining the protein with the rigid macroporous polymer.