USE AND MAKING OF BIOSENSORS UTILIZING ANTIMICROBIAL PEPTIDES FOR HIGHLY SENSITIVE BIOLOGICAL MONITORING
First Claim
1. A biosensor configured to detect a target, the biosensor comprising:
- a peptide immobilized on a sensing component, the sensing component having an anode and a cathode, the immobilized peptide comprising an antimicrobial peptide binding motif for the target, the sensing component having an electrical conductivity that changes in response to binding of the immobilized peptide to the target.
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Accused Products
Abstract
A biosensor and method of making are disclosed. The biosensor is configured to detect a target and may include a peptide immobilized on a sensing component, the sensing component having an anode and a cathode. The immobilized peptide may comprise an antimicrobial peptide binding motif for the target. The sensing component has an electrical conductivity that changes in response to binding of the immobilized peptide to the target. The immobilized peptide may bind one or more targets selected from the list consisting of: bacteria, Gram-negative bacteria, Gram-positive bacteria, pathogens, protozoa, fungi, viruses, and cancerous cells. The biosensor may have a display with a readout that is responsive to changes in electrical conductivity of the sensing component. The display unit may be wirelessly coupled to the sensing component. A resonant circuit with an inductive coil may be electrically coupled to the sensing component. A planar coil antenna may be disposed in proximity to the resonant circuit, the planar coil antenna being configured to provide power to the sensing component.
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Citations
36 Claims
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1. A biosensor configured to detect a target, the biosensor comprising:
a peptide immobilized on a sensing component, the sensing component having an anode and a cathode, the immobilized peptide comprising an antimicrobial peptide binding motif for the target, the sensing component having an electrical conductivity that changes in response to binding of the immobilized peptide to the target. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 20, 21, 22, 24, 25)
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19. A method of using an immobilized antimicrobial peptide to detect a target in a sample, the method comprising:
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exposing the sample to at least one peptide immobilized on a sensing component, the immobilized peptide having an antimicrobial binding motif, the electrical conductivity of the sensing component changing in response to binding of the immobilized peptide with a target; measuring the electrical conductivity of the sensing component; and determining the presence or absence of the target in the sample based on the electrical conductivity of the sensing component.
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23. A method of making a biosensor configured to detect a target, the method comprising:
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selecting an antimicrobial peptide based on its binding affinity for the target; immobilizing the antimicrobial peptide on a sensing component, the sensing component having an electrical conductivity that changes based on binding to the target; electrically coupling an anode and a cathode to the sensing component. - View Dependent Claims (26)
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27. A biosensor comprising:
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an antimicrobial peptide immobilized on a microbe, the microprobe having an electrical conductivity that is dependent on a binding state of the binding molecule; an anode and a cathode coupled to the microprobe; a display with a readout electrically coupled to the cathode and anode, forming a circuit, the readout being based on the electrical conductivity of the microprobe.
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28. A biosensor comprising:
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an interdigitated microelectrode array disposed on a bioresorbable substrate; an anode and a cathode electrically coupled to the interdigitated microelectrode array; a resonant circuit with an inductive coil electrically coupled to the cathode and anode; and a wireless telemetry unit electrically coupled to the resonant circuit, forming an electric circuit. - View Dependent Claims (29, 30, 31, 32, 33, 34, 35, 36)
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Specification