METHOD FOR MANUFACTURING MULTIPLE-DIAGNOSIS MEMBRANE SENSOR BY USING SCREEN PRINTING

• US 20140287439A1
• Filed: 10/05/2012
• Published: 09/25/2014
• Est. Priority Date: 10/06/2011
• Status: Active Grant

• Current Assignee: Ingibio Ltd.
• Original Assignee: Ingibio Ltd.
• Inventors: Kim, Min-Gon, Joung, Hyou-Arm, Ahn, Jun-Hyoung, Suk, Ho-Jun
• Granted Patent: US 9,970,933 B2
• Time in Patent Office: Days
• US Class Current: 435/7.72
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing
• CPC Class Codes: G01N 33/54388 based on lateral flow
• CPC Class Codes: G01N 33/558 using diffusion or migration of antigen or antibody immunochromatographic test strips G01N33/54387
• CPC Class Codes: G01N 33/56911 Bacteria
• CPC Class Codes: G01N 33/56916 Enterobacteria, e.g. shigella, salmonella, klebsiella, serratia

Abstract

Provided is a method for manufacturing a multiple-diagnosis membrane sensor provided with multiple channels by using screen printing, and more specifically, to a method for manufacturing a membrane sensor capable of performing multiple-diagnosis by screen-printing hydrophobic ink on a membrane to form multiple channels.

The membrane sensor according to the present invention may enable mass-production of sensors and secure reliability of detection by forming the plurality of channels on the membrane by a simple method.

Claims

1. A method for manufacturing a multiple channel membrane sensor, the method comprising:

screen printing hydrophobic ink onto a membrane in which a water soluble sample flows to form multiple channels.

2. The method of claim 1, wherein the membrane is made of one or more of nitrocellulose, nylon, polysulfone, polyethersulfone, polyvinylidene fluoride (PVDF).

3. The method of claim 1, wherein as the hydrophobic ink, one kind or more selected from a silver paste, a carbon paste, a platinum paste, a ceramic paste, and wax is used.

4. The method of claim 1, wherein the hydrophobic ink contains one kind or two kinds or more organic solvents selected from alcohols having 1 to 6 carbon atoms, dmethylformamide (DMF), dimethyl sulfoxide (DMSO), and acetone.

5. A membrane sensor using a lateral flow, the membrane sensor comprising:

multiple channels formed by screen printing hydrophobic ink on a surface of a membrane.

6. The membrane sensor of claim 5, further comprising a sample pad, a conjugation pad, an absorption pad on the membrane.

7. The membrane sensor of claim 6, wherein the sample pad or absorption pad is made of one kind or more selected from cellulose, polyester, polypropylene, and glass fiber.

8. The membrane sensor of claim 6, wherein the conjugation pad is made of one kind or more selected from cellulose, polyester, polypropylene, glass fiber, nitrocellulose, nylon, polysulfone, polyethersulfone, and polyvinylidene fluoride (PVDF).

9. The membrane sensor of claim 6, wherein the conjugation pad is treated with a signal generation material or a conjugate of a material selectively binding to an analyte and the signal generation material.

10. The membrane sensor of claim 9, wherein the signal generation material is metal nanoparticles, quantum dot nanoparticles, magnetic nanoparticles, an enzyme, an enzyme substrate, an enzyme reactant, a light absorbent, a fluorescent material, or a luminescent material.

11. A method of analyzing a biological sample characterized by injecting a sample into the membrane sensor of claim 5 to measure an analyte.

12. The method of analyzing a biological sample of claim 11, further comprising measuring electrochemical signals between multiple channels of the membrane sensor.