Rapid High-Resolution Imaging Methods for Large Samples
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Accused Products
Abstract
Light Sheet Theta (LS-0) Microscopy achieves large sample imaging capabilities without affecting the imaging depth or the image quality. An optical layout places a detection objective normal to the sample surface, while placing the illumination objectives that generate light sheets at an angle (theta) significantly smaller than 90 degrees. In this configuration, the light sheets enter from same side of the sample as the detection objective. The intersection of the light-sheet and the detection focal plane results in a line illumination-detection profile that is discriminated by a camera.
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Citations
45 Claims
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1-3. -3. (canceled)
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4. A microscope, comprising:
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an imaging component, with an imaging objective, and a first light sheet projector located on a same side of a sample region; the imaging objective and first light sheet projector having respective axes that intersect in the sample region; a sample support positioned to hold a sample in said sample region; the light sheet projector having light scanning and focusing optics to permit the selection of an axial position of a light sheet beam waist and the selection of a position of the light sheet along an axis perpendicular to the light sheet propagation direction; a controller connected to control the light scanning and focusing optics to translate the beam waist along a linear path; the imaging objective optical axis being perpendicular to said linear path. - View Dependent Claims (5, 6, 7, 8, 9)
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10-14. -14. (canceled)
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16-30. -30. (canceled)
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31. An imaging instrument, comprising:
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a microscope with an objective; a first light beam projector with imaging optics separate from said microscope positioned to direct a beam of light that crosses said optical axis; and the first light beam projector forming a sheet having a beam waist in the form of a line of maximum intensity; the first light beam projector having a scanning mechanism that scans the line of maximum intensity through a range of positions along a scan direction perpendicular to the line of maximum intensity. - View Dependent Claims (15, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 44)
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43. (canceled)
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45-114. -114. (canceled)
Specification