Dry deposition of materials for microarrays using matrix displacement

• US 6,653,151 B2
• Filed: 01/31/2001
• Issued: 11/25/2003
• Est. Priority Date: 07/30/1999
• Status: Expired due to Fees

• Current Assignee: Large Scale Proteomics Corporation
• Original Assignee: Large Scale Proteomics Corporation
• Inventors: Braatz, James A., Anderson, N. Leigh, Anderson, Norman G.
• Primary Examiner(s): Le, Long V.
• Assistant Examiner(s): COUNTS, GARY W
• Application Number:
  US09/772,974
• Publication Number:
  US 20010012537A1
• Time in Patent Office: 1,028 Days
• Field of Search: 435/6, 435/7.1, 436/501, 436/518, 436/524, 427/163.2, 427/256, 427/287, 427/429, 427/275, 427/399, 427/2.11
• US Class Current: 506/32
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00515 in the shape of strings
• CPC Class Codes: B01J 2219/0052 in the shape of elongated tubes
• CPC Class Codes: B01J 2219/00524 in the shape of fiber bundles
• CPC Class Codes: B01J 2219/00547 Bar codes
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00617 by chemical means
• CPC Class Codes: B01J 2219/00619 using hydrophilic or hydrophobic regions
• CPC Class Codes: B01J 2219/00621 by physical means, e.g. trenches, raised areas
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/0063 Other, e.g. van der Waals forces, hydrogen bonding
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00641 the porous medium being continuous, e.g. porous oxide substrates
• CPC Class Codes: B01J 2219/00644 the porous medium being present in discrete locations, e.g. gel pads
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00664 Three-dimensional arrays
• CPC Class Codes: B01J 2219/00673 : Slice arrays
• CPC Class Codes: B01J 2219/00707 : separated from the reactor apparatus
• CPC Class Codes: B01J 2219/0072 : Organic compounds
• CPC Class Codes: B01J 2219/00722 : Nucleotides
• CPC Class Codes: B01J 2219/00731 : Saccharides
• CPC Class Codes: B01L 3/50857 : using arrays or bundles of open capillaries for holding samples
• CPC Class Codes: C12Q 1/6837 : using probe arrays or probe chips C12Q1/6874 takes precedence
• CPC Class Codes: C40B 40/06 : Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 40/12 : Libraries containing saccharides or polysaccharides, or derivatives thereof
• CPC Class Codes: C40B 70/00 : Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or bar codes
• CPC Class Codes: G01N 2500/00 : Screening for compounds of potential therapeutic value
• CPC Class Codes: G01N 33/54313 : the carrier being characterised by its particulate form
• CPC Class Codes: G01N 33/5436 : with ligand physically entrapped within the solid phase liposomes G01N33/5432; immunological test elements G01N33/54386
• CPC Class Codes: G01N 33/54366 : Apparatus specially adapted for solid-phase testing
• CPC Class Codes: Y10T 428/29 : Coated or structually defined flake, particle, cell, strand, strand portion, rod, filament, macroscopic fiber or mass thereof

Abstract

A method is disclosed which relates to the placement of binding partners on microarrays, where such binding partners contain proteins, nucleic acids, biological cells and other bio-reactive components. The present invention discloses uses and methods for manufacture of microarrays constructed in part by sectioning bundles of tubules or rods containing matrix immobilized bio-reactive molecules to produce large numbers of sample chips. The chips so produced are processed by deposition to microarrays. The deposited chips can then be manipulated to partition the immobilizing matrix away from the bio-reactive molecules contained in the matrix and to place said partitioned molecules onto various surfaces for subsequent analysis, to include binding assays, hybridization reactions, diagnostic methods and a variety of cell interaction-determining methodologies.

Claims

1. A method of dry dispensing a binding molecule in a defined area on a surface comprising:

(1) placing a solid in said area on said surface without the use of a liquid vehicle, wherein said solid comprises a matrix and said binding molecule, wherein said binding molecule is entrapped in said matrix, and wherein each of a plurality of defined areas comprises a different binding molecule;

(2) degrading said matrix of step (1);

(3) substantially removing degraded matrix from said surface to allow said binding molecule to be deposited in said defined area; and

(4) retaining said binding molecule on said surface, wherein said retaining step uniformly adheres said binding molecule to said defined area on said surface.

2. The method of claim 1, wherein said solid is formed from a transversely sliced section of a fiber.

3. The method of claim 2, wherein at least 100 fibers are bundled in a fixed alignment with respect to each other.

4. The method of claim 1, wherein said matrix is selected from the group consisting of super-cooled liquid, crystal, plastic, and colloid suspension.

5. The method of claim 1, wherein said entrapped binding molecule is a biological macromolecule.

6. The method of claim 5, wherein said macromolecules are selected from the group consisting of proteins, carbohydrates, nucleic acids, and lipids.

7. The method of claim 1, wherein said solid is positioned on at least one porous membrane which abuts said surface or is said surface, before degrading said matrix.

8. The method of claim 7, wherein the matrix is removed by passage through the porous membrane.