Apparatus and method for imaging
First Claim
1. A cassette for use with an imaging apparatus, said cassette comprising:
- an optical element comprising a reflective surface to allow formation thereon of a specimen array, the optical element being configured to direct a polarized light beam from a light source to the reflective surface, such that a reflection of the polarized light beam at the reflective surface generates an evanescent field in the vicinity of the specimen array; and
a mount or frame coupled to the optical element and having a locating portion engageable with a mating locating element of a two-dimensional array detector adapted for providing an image based on spatially distributed polarization changes in the light beam.
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Accused Products
Abstract
Apparatus for acquiring an image of a specimen comprising a cassette having an optical portion holding a specimen array on a TIR surface and being removably matable to a processing portion having a polarized light beam source and a processing polarization-sensitive portion to image the spatially distributed charges in polarization of the specimen array. In one form the array optical portion comprises a transparent slide having a bottom surface with first and second gratings located to direct polarized light to the TIR surface and to direct light reflected by that (TIR) surface to an imager, respectively. The apparatus may include a flow cell integral with the optical portion as well as means for selecting the direction and wavelength of the polarized light.
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Citations
23 Claims
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1. A cassette for use with an imaging apparatus, said cassette comprising:
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an optical element comprising a reflective surface to allow formation thereon of a specimen array, the optical element being configured to direct a polarized light beam from a light source to the reflective surface, such that a reflection of the polarized light beam at the reflective surface generates an evanescent field in the vicinity of the specimen array; and
a mount or frame coupled to the optical element and having a locating portion engageable with a mating locating element of a two-dimensional array detector adapted for providing an image based on spatially distributed polarization changes in the light beam. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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12. A cassette for use with an imaging apparatus, said cassette comprising:
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an optical element including a prism and a slide having a reflective surface to allow formation thereon of a specimen array, the optical element being configured to direct a polarized light beam from a light source to the reflective surface, such that a reflection of the polarized light beam at the reflective surface generates an evanescent field in the vicinity of the specimen array; and
a mount or frame coupled to the optical element and having a locating portion engageable with a mating locating element of a two-dimensional array detector adapted for providing an image based on spatially distributed polarization changes in the light beam, the mount or frame being operable to locate at least a selected portion of the specimen array in the evanescent field. - View Dependent Claims (13)
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14. A cassette for use with an imaging apparatus, said cassette comprising:
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an optical element comprising a reflective surface to allow formation thereon of a specimen array, said optical element being configured to direct a polarized light beam from a light source to the reflective surface, such that a reflection of the polarized light beam at the reflective surface generates an evanescent field in the vicinity of the specimen array; and
a mount or frame coupled to the optical element and having a locating portion engageable with a mating locating element of a processing assembly. - View Dependent Claims (15, 16, 17, 18, 19, 20, 21, 22, 23)
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Specification