Optical bio-discs and fluidic circuits for analysis of cells and methods relating thereto
First Claim
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1. An optical bio-disc for conducting a cellular assay, said disc comprising:
- a rotatable substrate;
a cap portion associated with said substrate; and
a fluidic circuit formed between said substrate and said cap portion, said fluidic circuit comprising;
a mixing chamber, said mixing chamber including an inlet port configured to receive a fluid including RBC'"'"'s;
a RBC capture zone in fluid communication with the mixing chamber so as to receive a fluid therefrom;
a plurality of posts with RBC capture agents coated thereon and located within the RBC capture zone, the posts being configured to capture RBC'"'"'s while allowing passage through said RBC capture zone of non-RBC materials included in the fluid;
a purification chamber in fluid communication with said capture zone, the purification chamber configured to receive mixed fluid communicated from the mixing chamber;
a filter associated with said purification chamber;
an analysis chamber in fluid communication with said purification chamber, said analysis chamber including a capture zone; and
a vent port associated with said analysis chamber.
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Abstract
The present invention is directed to optical bio-discs, methods, and optical disc drives for clinical diagnostics. The present invention is further directed to methods for determining the quantity of a specific type of blood cell in a biological sample including on-disc sample preparation and processing, providing a pre-processed sample onto a capture zone, removing portions of the sample that are not bound in the capture zone, and counting bound cells. Also disclosed are fluidic circuits for processing blood samples for use in a cluster designation marker assay in the optical bio-disc.
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Citations
21 Claims
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1. An optical bio-disc for conducting a cellular assay, said disc comprising:
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a rotatable substrate; a cap portion associated with said substrate; and a fluidic circuit formed between said substrate and said cap portion, said fluidic circuit comprising; a mixing chamber, said mixing chamber including an inlet port configured to receive a fluid including RBC'"'"'s; a RBC capture zone in fluid communication with the mixing chamber so as to receive a fluid therefrom; a plurality of posts with RBC capture agents coated thereon and located within the RBC capture zone, the posts being configured to capture RBC'"'"'s while allowing passage through said RBC capture zone of non-RBC materials included in the fluid; a purification chamber in fluid communication with said capture zone, the purification chamber configured to receive mixed fluid communicated from the mixing chamber; a filter associated with said purification chamber; an analysis chamber in fluid communication with said purification chamber, said analysis chamber including a capture zone; and a vent port associated with said analysis chamber. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 20, 21)
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16. A method of making an optical bio-disc for use in cellular assays, said method of making comprising the steps of:
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forming a rotatable optical disc substrate; forming a cover disc with similar dimensions to said substrate; forming a channel layer with cut out portions to form fluidic circuits;
said fluidic circuits including an inlet port, a mixing chamber, a purification chamber, an analysis chamber, a vent port;
a RBC capture zone in fluid communication with the mixing chamber so as to receive a fluid therefrom; and
a plurality of posts with RBC capture agents coated thereon and located within the RBC capture zone;
said posts configured to capture RBC'"'"'s while allowing passage through said RBC capture zone of non-RBC materials; and
said analysis chamber including capture zones;binding capture probes onto said capture zones; attaching said channel layer to said substrate; loading a filter means into said purification chamber; attaching said cover disc to said channel layer to thereby form the optical bio-disc. - View Dependent Claims (17, 18, 19)
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Specification