Electrically non-conductive, nanoparticulate membrane

• US 7,563,588 B2
• Filed: 10/25/2004
• Issued: 07/21/2009
• Est. Priority Date: 10/29/2003
• Status: Expired due to Fees

• Current Assignee: Agency For Science Technology and Research (Government of Singapore)
• Original Assignee: Agency For Science Technology and Research (Government of Singapore)
• Inventors: Xie, Fang, Ying, Yi-Ru Jackie, Mohamed Arshad, Mohamed Shariff, Xu, Guolin, Gao, Zhiqiang
• Primary Examiner(s): GITOMER, RALPH J
• Application Number:
  US10/577,292
• Publication Number:
  US 20070042377A1
• Time in Patent Office: 1,730 Days
• Field of Search: 435/14
• US Class Current: 435/14
• CPC Class Codes: B01D 69/14111 with nanoscale dispersed material, e.g. nanoparticles
• CPC Class Codes: C12N 9/0006 acting on CH-OH groups as donors (1.1)
• CPC Class Codes: C12Q 1/002 Electrode membranes
• CPC Class Codes: C12Q 1/003 Functionalisation
• CPC Class Codes: C12Q 1/004 mediator-assisted
• CPC Class Codes: C12Q 1/005 involving specific analytes or enzymes including groups of enzymes, e.g. oxydases; C12Q1/004 takes precedence
• CPC Class Codes: C12Q 1/26 involving oxidoreductase
• CPC Class Codes: C12Y 101/03004 Glucose oxidase (1.1.3.4)
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52
• CPC Class Codes: G01N 33/5438 Electrodes

Abstract

An electrically non-conductive, nanoparticulate membrane comprising nanoparticles of at least one inorganic oxide of an element selected from Group IA, IIA, IIIA, IVA, IB, IIB, IIIB, IVAB, VB, VIB, VIIB or VIIIB of the Periodic Table, and wherein an oxidoreductase enzyme and a polymeric redox mediator capable of transferring electrons are diffusibly dispersed in said nanoparticulate membrane.

Claims

1. An electrically non-conductive, nanoparticulate membrane comprising nanoparticles of at least one inorganic oxide of an element selected from Group IA, IIA, IIA, IVA, IB, IIB, IIIB, IVAB, VB, VIB, VIIB, VIIIB of the Periodic Table, and wherein an oxidoreductase enzyme and polymeric redox mediator capable of transferring electrons are diffusibly dispersed in said nanoparticulate membrane to allow diffusion of the oxidoreductase enzyme, the polymeric redox mediator and analyte within the membrane when the membrane is exposed to a test sample containing said analyte.

2. The membrane according to claim 1, wherein the oxidoreductase is selected from the group consisting of glucose oxidase, hydrogen peroxidase, horseradish peroxidase, xanthine oxidase, cholesterol oxidase, hydrogen hydrogenase, lactate dehydrogenase, glucose dehydrogenase, NADH dehydrogenase, sarcosine oxidase, lactate oxidase, alcohol dehydrogenase, hydroxybutyrate dehydrogenase, glycerol dehydrogenase, sorbitol dehydrogenase, malate dehydrogenase, galactose dehydrogenase, malate oxidase, galactose oxidase, xanthine dehydrogenase, alcohol oxidase, choline oxidase, xanthine oxidase, choline dehydrohenase, pyruvate dehydrogenase, pyruvate oxidase, oxalate oxidase, bilirubin oxidase, glutamate dehydrogenase, glutamate oxidase, amine oxidase, NADPH oxidase, urate oxidase, cytochrome C oxidase, and actechol oxidase.

3. The membrane of claim 1, wherein the polymeric redox mediator capable of transferring electrons is a vinylferrocene-based polymeric redox mediator capable of transferring electrons.

4. The membrane according to claim 1, wherein the oxidoreductase is covalently linked to the polymeric redox mediator by cross-linkages.

5. The membrane of claim 1, wherein the element selected from Group IA, IIA, IIIA, IVA, IB, IIB, IIIB, IVAB, VB, VIB, VIIB or VIIIB of the Periodic Table is selected from the group consisting of aluminium, silicon, magnesium and zinc.

6. The membrane of claim 1, wherein the thickness of the membrane ranges from 250 to 500 μ

m.

7. The membrane of claim 1, wherein the size of the nanoparticles ranges from 10 nm to 1 μ

m.

8. The membrane of claim 1, wherein the membrane further comprises a polymeric binder.

9. The membrane according to claim 8, wherein the polymeric binder is a polymer or copolymer comprising monomer units selected from the group consisting of vinyl pyridine, vinyl imidazole, acrylamide, acrylonitrile, and acrylhydrazide and acrylic acid.

10. The membrane according to claim 1, wherein the membrane is adapted for determination of glucose concentration.

11. The membrane according to claim 3, wherein the vinylferrocene-based polymeric redox mediator is selected from the group consisting of poly(vinyl ferrocene), poly(vinyl ferrocene)-co-acrylamide, poly(vinyl ferrocene)-co-acrylic acid, and poly(vinyl ferrocene)-co-acrylamido-(CH₂)_n-sulfonic acid, and poly(vinyl ferrocene)-co-acrylamido-(CH₂)_n-phosphonic acid, wherein n is an integer from 0 to 12.