Detection of analytes using reorganization energy

• US 7,566,534 B2
• Filed: 10/30/2007
• Issued: 07/28/2009
• Est. Priority Date: 06/12/1997
• Status: Expired due to Fees

• Current Assignee: Osmetech Technology Inc. (Roche Holding AG)
• Original Assignee: Osmetech Technology Inc. (Roche Holding AG)
• Inventors: Meade, Thomas J.
• Primary Examiner(s): Monshipouri; Maryam
• Application Number:
  US11/978,931
• Publication Number:
  US 20080171340A1
• Time in Patent Office: 637 Days
• Field of Search: 435/5, 435/6, 435 7- 9, 435/7.91, 205/793.5, 205/777.5, 205/778
• US Class Current: 435/5
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: B82Y 5/00 Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
• CPC Class Codes: G01N 33/542 with steric inhibition or signal modification, e.g. fluorescent quenching
• CPC Class Codes: G01N 33/5438 Electrodes

Abstract

The invention relates to novel methods and compositions for the detection of analytes using the nuclear reorganization energy, λ, of an electron transfer process.

Claims

1. A method of detecting a target analyte in a test sample, said method comprising:

(1) adding a target analyte to an electrode comprising a redox active complex, said redox active complex comprising;

(i) a first species comprising a redox active molecule, said redox active molecule comprising at least one polar ligand and a metal selected from the group consisting of manganese, technetium, rhenium, iron, ruthenium, osmium, cobalt, rhodium, iridium, nickel, palladium, platinum, copper, silver and gold;

(ii) a second species comprising a binding ligand that will bind to said target analyte, such that upon binding of said target analyte to said binding ligand, there is a change of the E⁰ of said redox active molecule,

wherein said first species and said second species sterically close to the first species are attached separately to said electrode; and

(2) measuring said change in the E⁰ of said redox active complex.

2. The method according to claim 1, wherein said metal is osmium.

3. The method according to claim 1, wherein said redox active molecule comprises at least one polar ligand.

4. The method according to claim 3, wherein said polar ligand is a small polar ligand.

5. The method according to claim 1, wherein said redox active molecule comprises a cyano group.

6. The method according to claim 1, wherein said binding ligand is a protein.

7. The method according to claim 6, wherein said protein is a peptide.

8. The method according to claim 1, wherein said binding ligand is a substrate of an enzyme.

9. The method according to claim 1, wherein said attachment is via a spacer.

10. The method according to claim 9, wherein said spacer is an insulator.

11. The method according to claim 9, wherein said spacer is a conductive oligomer.

12. The method according to claim 1, wherein said measuring step is carried out using voltammetry.