Frequency-multiplexed detection of multiple wavelength light for flow cytometry
First Claim
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1. A multiple wavelength optical system comprising:
- a plurality of light sources;
a frequency modulator connected to each light source;
a dichroic fold mirror having an element proximate to each light source, and an output of light directed to a target;
an array of annular light detectors proximate to the target; and
a frequency analyzer connected to the light detector;
wherein the annular light detectors are isolated from each other by an annular area that is not sensitive to light such that each annular detector subtends its own prescribed angle and the annular detectors provide an electrical signal representing light impinging the detector at respective angles.
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Abstract
A multiplexed set of light sources having outputs of light with various wavelengths which are combined into one beam. The beam may impinge a particle in a flow channel of a cytometer. The light leaving the flow channel may be sensed by a detector and the light distinguished according to wavelength.
101 Citations
18 Claims
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1. A multiple wavelength optical system comprising:
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a plurality of light sources; a frequency modulator connected to each light source; a dichroic fold mirror having an element proximate to each light source, and an output of light directed to a target; an array of annular light detectors proximate to the target; and a frequency analyzer connected to the light detector; wherein the annular light detectors are isolated from each other by an annular area that is not sensitive to light such that each annular detector subtends its own prescribed angle and the annular detectors provide an electrical signal representing light impinging the detector at respective angles. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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12. A method for identifying components of a detected light beam having different wavelengths, comprising:
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modulating with a first frequency a first light having a first wavelength; modulating with another frequency at least another light having another wavelength; combining the light having the first wavelength with the at least another light with the another wavelength into a light beam; detecting the light beam with an array of light detectors that converts the detected light into an electrical signal representing light impinging the detector at respective angles and are separated from one another by an area that is not sensitive to light such that each detector subtends its own prescribed angle; and analyzing the electrical signal into signals representing light of the first wavelength and signals representing the at least another light having another wavelength. - View Dependent Claims (13, 14, 15, 16, 17, 18)
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Specification