Benzoate inductible promoters

• US 7,705,203 B2
• Filed: 11/12/2003
• Issued: 04/27/2010
• Est. Priority Date: 11/12/2002
• Status: Active Grant

• Current Assignee: Purdue Research Foundation (Purdue University)
• Original Assignee: Purdue Research Foundation (Purdue University)
• Inventors: Hodges, Thomas K., Antunes, Mauricio S., Carpita, Nicholas
• Primary Examiner(s): Kubelik; Anne R
• Application Number:
  US10/534,405
• Publication Number:
  US 20060277620A1
• Time in Patent Office: 2,358 Days
• Field of Search: None
• US Class Current: 800/298
• CPC Class Codes: C12N 15/8238 chemically inducible, e.g. tetracycline
• CPC Class Codes: C12N 15/827 Flower development or morphology, e.g. flowering promoting factor [FPF]

Abstract

Benzoate inducible promoters and promoter systems are disclosed, and uses thereof. Polynucleotides disclosing Benzoate Response Elements are also disclosed.

Claims

1. A recombinant plant expression vector comprising:

a) a benzoate inducible promoter comprising a benzoate response element comprising a fragment of SEQ ID NO;

1 comprising SEQ ID NO;

4 operably linked to a promoter functional in a plant, and

b) a nucleic acid sequence of interest, wherein said nucleic acid sequence of interest is operably linked to said benzoate inducible promoter.

2. The recombinant plant expression vector of claim 1, wherein said promoter comprises a plant minimal gene promoter.

3. The recombinant plant expression vector of claim 1, wherein said promoter is 35S.

4. The recombinant plant expression vector of claim 1, wherein the fragment of SEQ ID NO:

1 comprising SEQ ID NO;

4 is SEQ ID NO;

1, SEQ ID NO;

2, SEQ ID NO;

3, SEQ ID NO;

4, or SEQ ID NO;

6.

5. A transgenic plant cell comprising:

a) a benzoate inducible promoter comprising a benzoate response element comprising a fragment of SEQ ID NO;

1 comprising SEQ ID NO;

4 operably linked to a promoter functional in a plant, and

b) a nucleic acid sequence of interest, wherein said nucleic acid sequence of interest is operably linked to said benzoate inducible promoter.

6. The transgenic plant cell of claim 5, wherein said promoter comprises a plant minimal gene promoter.

7. The transgenic plant cell of claim 5, wherein said promoter is 35S.

8. The transgenic plant cell of claim 5, wherein the fragment of SEQ ID NO:

1 comprising SEQ ID NO;

4 is SEQ ID NO;

1, SEQ ID NO;

2, SEQ ID NO;

3, SEQ ID NO;

4, or SEQ ID NO;

6.

9. A transgenic plant cell comprising:

a) a benzoate inducible promoter comprising a benzoate response element comprising a fragment of SEQ ID NO;

1 comprising SEQ ID NO;

4 operably linked to a promoter functional in a plant, and

b) a nucleic acid sequence of interest, wherein said nucleic acid sequence of interest is operably linked to said benzoate inducible promoter.

10. The transgenic plant cell of claim 9, wherein said promoter comprises a plant minimal gene promoter.

11. The transgenic plant cell of claim 9, wherein said promoter is 35S.

12. The transgenic plant cell of claim 9, wherein the fragment of SEQ ID NO:

1 comprising SEQ ID NO;

4 is SEQ ID NO;

1, SEQ ID NO;

2, SEQ ID NO;

3, SEQ ID NO;

4, or SEQ ID NO;

6.

13. A method of controlling expression of a nucleic acid sequence of interest in a plant, the method comprising:

a) transforming said plant with an expression cassette comprising

i) a benzoate inducible promoter comprising a benzoate response element comprising a fragment of SEQ ID NO;

1 comprising SEQ ID NO;

4 operably linked to a promoter functional in a plant, and

ii) a nucleic acid sequence of interest, wherein said nucleic acid sequence of interest is operably linked to said benzoate inducible promoter; and

b) contacting said transformed plant with benzoate, whereby said benzoate inducible promoter in the presence of said benzoate induces the expression of said nucleic acid sequence of interest.

14. The method of claim 13, wherein said promoter comprises a plant minimal gene promoter.

15. The method of claim 13, wherein said promoter is 35S.

16. The method of claim 13, wherein the fragment of SEQ ID NO:

1 comprising SEQ ID NO;

4 is SEQ ID NO;

1, SEQ ID NO;

2, SEQ ID NO;

3, SEQ ID NO;

4, or SEQ ID NO;

6.