Biomolecule detection device, mobile phone for biomolecule detection, and biomolecule detection method

• US 7,828,949 B2
• Filed: 12/06/2006
• Issued: 11/09/2010
• Est. Priority Date: 12/08/2005
• Status: Expired due to Fees

• Current Assignee: Electronics and Telecommunications Research Institute
• Original Assignee: Electronics and Telecommunications Research Institute
• Inventors: Jung, Moon Youn, Park, Se Ho, Shin, Dong Ho, Pyo, Hyeon Bong
• Primary Examiner(s): Neckel; Alexa D
• Assistant Examiner(s): DIETERLE, JENNIFER M
• Application Number:
  US11/634,710
• Publication Number:
  US 20070131552A1
• Time in Patent Office: 1,434 Days
• Field of Search: 455/73, 455/91, 455/400, 455/403, 204/459, 204/610, 204/456, 204/606, 204/450, 204/451, 204/466, 204/601, 204/605, 204/612, 600/300, 600/301, 600309-310, 600/316, 600/322, 600/347, 600/365, 600/481, 600/500, 600/587, 600/595, 128903-904, 702/19, 702/104, 434235-238, 434/262, 273/429, 273/440, 273/459, 273/148.B, 382/313, 435/6, 435/7.1, 435/287.2
• US Class Current: 204/456
• CPC Class Codes: A61B 5/0002 Remote monitoring of patients using telemetry, e.g. transmission of vital signals via a communication network A61B5/07 takes precedence; transmission systems for measured values G08C; transmission H04B; transmission of digital information H04L; wireless communication networks H04W
• CPC Class Codes: G01N 27/44704 Details; Accessories
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: G01N 33/544 the carrier being organic
• CPC Class Codes: G01N 33/548 Carbohydrates, e.g. dextran

Abstract

Provided are a biomolecule detection device, a mobile phone for biomolecule detection, and a biomolecule detection method. The biomolecule detection device includes an electrophoresis unit comprising an electrophoretic gel filtering erythrocytes and leukocytes in blood and transferring proteins and DNAs in the blood, and at least one type of a probe biomolecule, immobilized in the electrophoretic gel, reacting with a target biomolecule; a conversion unit converting a result of a reaction between the target biomolecule and the probe biomolecule to an electrical signal; and a lead-out unit receiving, converting, and transmitting the electrical signal.

Claims

1. A portable handheld wireless device for biomolecule detection comprising:

an electrophoresis unit comprising;

a sample inlet;

a filter in fluid communications with the sample inlet;

an electrophoretic gel in fluid communications with the filter;

a plurality of immobilization regions serially spaced apart from one another along a separation pathway within the electrophoretic gel; and

opposing electrodes for providing an electric field that defines the separation pathway through the electrophesis gel unit and through the immobilization regions;

an optical source irradiating light to the electrophoresis unit, wherein the electrophoresis unit is sized, shaped, and arranged on the portable handheld wireless device so as to receive light irradiating from a screen of the portable handheld wireless device during operation;

a conversion unit comprising a plurality of detectors optically aligned with corresponding immobilization regions in the electrophoretic gel in which the optically aligned detectors are configured to physically respond to changes in light that transmits through the immobilization regions and strikes onto corresponding optically aligned detectors which in turn drives production of corresponding electrical signals; and

a lead-out unit receiving, converting, and transmitting the electrical signals.

2. The portable handheld wireless device of claim 1, wherein each immobilization region comprises a glass fiber or a polymer material which is disposed in the electrophoretic gel and which has a probe biomolecule immobilized onto each immobilization region.

3. The portable handheld wireless device of claim 1, wherein the electrophoretic gel is an agarose gel or a polyacrylamide gel.

4. The portable handheld wireless device of claim 1 wherein the portable handheld wireless device comprises a mobile phone.

5. The portable handheld wireless device of claim 1, wherein the plurality of immobilization regions comprises a plurality of antibody immobilization regions.

6. The portable handheld wireless device of claim 5, wherein the plurality of immobilization regions comprises a first antibody immobilization region, a second antibody immobilization region, and a third antibody immobilization region.

7. The portable handheld wireless device of claim 6, wherein the first antibody immobilization region having a first antibody affixed onto the first antibody immobilization region, the second immobilization region having a second antibody affixed onto the second antibody immobilization region, and the third antibody immobilization region having a third antibody affixed onto the third antibody immobilization region.

8. The portable handheld wireless device of claim 1, wherein the immobilization regions comprise nucleic acid immobilization regions having a probe nucleic acid affixed onto the nucleic acid immobilization regions.

9. The portable handheld wireless device of claim 8, wherein the probe nucleic acid is selected from the group consisting of DNAs, RNAs, PNAs, LNAs, and hybrids thereof.

10. The portable handheld wireless device of claim 8, wherein the immobilization regions comprise protein immobilization regions having a probe protein affixed onto the protein acid immobilization regions such that the probe protein is selected from the group consisting of enzymes, substrates, antigens, antibodies, ligands, aptamers, and receptors.

11. The portable handheld wireless device of claim 1, wherein the electrophoresis unit further comprises two substrates facing each other, and having the electrophoretic gel and the immobilization regions interposed between the two substrates.

12. The portable handheld wireless device of claim 11, wherein the two substrates are transparent.

13. The portable handheld wireless device of claim 1, wherein the optically aligned detectors of the conversion unit are photodiodes.

14. The portable handheld wireless device of claim 13, wherein the photodiodes are capable of detecting visible light or UV light.

15. The portable handheld wireless device of claim 1, further comprising:

a power supply unit supplying power to the electrophoresis unit, the conversion unit, and the lead-out unit;

the optical source configured to irradiate light through the immobilization regions and onto the detectors of the electrophoresis unit; and

a transmitter configured to transmit wirelessly information corresponding to the electrical signals received from the lead-out unit.

16. The portable handheld wireless device of claim 15, wherein the optical source is an LCD screen of a mobile phone.

17. The portable handheld wireless device of claim 15, wherein the optical source comprises a UV light source.

18. A method of detecting a target biomolecule comprising:

obtaining a portable handheld wireless device for biomolecule detection comprising;

an electrophoresis unit comprising;

a sample inlet;

a filter in fluid communications with the sample inlet;

an electrophoretic gel in fluid communications with the filter;

a plurality of immobilization regions serially spaced apart from one another and positioned along a separation pathway within the electrophoretic gel wherein the immobilization regions comprise a first, second and third immobilization regions; and

opposing electrodes for providing an electric field that defines the separation pathway through the electrophoresis gel unit and through the immobilization regions;

an optical source irradiating light to the electrophoresis unit, wherein the electrophoresis unit is sized, shaped, and arranged on the portable handheld wireless device so as to receive light irradiating from a screen of the portable handheld wireless device during operation;

a conversion unit comprising a plurality of detectors optically aligned with corresponding immobilization regions in the electrophoretic gel in which the optically aligned detectors are configured to physically respond to changes in light that transmits through the immobilization regions and strikes onto corresponding optically aligned detectors which in turn drives production of corresponding electrical signals;

a lead-out unit receiving, converting, and transmitting the electrical signals,

a power supply unit supplying power to the electrophoresis unit, the conversion unit, and the lead-out unit;

an optical source configured to irradiate light through the immobilization regions and onto the detectors of the electrophoresis unit; and

a transmitter configured to transmit wirelessly information corresponding to the electrical signals received from the lead-out unit;

loading a sample into the sample inlet of the electrophoresis unit;

applying an electric field across the opposing electrodes to define the separation pathway through the electrophoresis gel unit and through the immobilization regions to promote separation of individual components of the sample along the separation pathway;

trapping and concentrating first, second and third target analytes at respective first, second and third immobilization regions when the electric field is applied across the opposing electrodes;

irradiating light from an optical source through the first, second, and third target immobilization regions when the first, second and third target analytes are respectively trapped and concentrated at the first, second and third immobilization regions;

detecting light through the first, second, and third immobilization regions when the first, second and third target analytes are trapped and concentrated at respective first, second and third immobilization regions by using the optically aligned detectors to respectively produce first, second and third electrical signals; and

transmitting first, second, and third wireless signals corresponding to the respective first, second and third electrical signals.

19. The method of claim 18, further comprising receiving the first, second, and third wireless signals at a remote terminal.

20. The method of claim 18, wherein the sample is selected from the group consisting of saliva, urine, blood, serum, cell culture, and drinking water.