Micro- or nano-fluidic chip fabricated with Norland Optical Adhesive and bioanalysis platform produced by using the same

• US 7,981,237 B2
• Filed: 10/12/2007
• Issued: 07/19/2011
• Est. Priority Date: 10/13/2006
• Status: Active Grant

• Current Assignee: EWHA University-Industry Collaboration Foundation (Ewha Womans University)
• Original Assignee: EWHA University-Industry Collaboration Foundation (Ewha Womans University)
• Inventors: Lee, Kang Mu, Kim, So Hyun, Kim, Youn Sang, Nam, Seong Won, Park, Sung Su
• Primary Examiner(s): Bullock; In Suk
• Assistant Examiner(s): Pregler; Sharon
• Application Number:
  US11/871,227
• Publication Number:
  US 20080253929A1
• Time in Patent Office: 1,376 Days
• Field of Search: 422/68.1, 422/99, 422/100, 422/102, 422/104, 422/130, 435/6, 216/24, 216/33, 216/37, 156/246, 156/261, 156/510, 436/529, 436/43, 264/248, 264/250, 264/331.19, 264/483, 264/494
• US Class Current: 156/246
• CPC Class Codes: B01L 2200/0668 Trapping microscopic beads
• CPC Class Codes: B01L 2200/0689 Sealing
• CPC Class Codes: B01L 2300/0825 Test strips
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B29C 59/14 by plasma treatment plasma tubes per se H01J
• CPC Class Codes: Y10T 156/107 Punching and bonding pressure application by punch
• CPC Class Codes: Y10T 156/12 Surface bonding means and/or assembly means with cutting, punching, piercing, severing or tearing
• CPC Class Codes: Y10T 436/11 Automated chemical analysis

Abstract

A micro- or nano-fluidic chip fabricated with a Norland Optical Adhesive (NOA), an acrylated polyurethane-based UV-polymerizable optical adhesive. The micro- or nano-fluidic chip has sequentially an inlet, a channel, and an outlet. The channel has a pillar in the region of the outlet to prevent beads from flowing out, and the surface of the channels in the micro- or nano-fluidic chip is hydrophilic, which generates spontaneous flow in the channels by a capillary force without any extra external pumping.

Claims

1. A method for fabricating a micro- or nano-fluidic chip, the method comprising the steps of:

i) fabricating a channel layer consisting of an acrylated polyurethane-based UV-polymerizable optical adhesive and having sequentially an inlet, a channel, and an outlet by pouring the acrylated polyurethane-based UV-polymerizable optical adhesive over a master mold and curing the acrylated polyurethane-based UV-polymerizable optical adhesive;

ii) fabricating a top cover layer by preparing a film having holes corresponding to the inlet and the outlet of the channel, coating the acrylated polyurethane-based UV-polymerizable optical adhesive on the film and curing the acrylated polyurethane-based UV-polymerizable optical adhesive; and

iii) bonding the channel layer obtained from step i) and the acrylated polyurethane-based UV-polymerizable optical adhesive coating of the top cover layer obtained from step ii) on a hot plate and curing the channel layer and the acrylated polyurethane-based UV-polymerizable optical adhesive coating under ultra-violet light,

wherein the channel layer surface is treated with oxygen plasma after step i).

2. The method of claim 1, wherein the film in the step ii) is a polyethylene terephthalate film.

3. The method of claim 1, wherein the channel has a pillar in the region of the outlet to prevent a plurality of beads from flowing out.

4. The method of claim 1, wherein the channel layer is a single layer.