Biosensor, thin film electrode forming method, quantification apparatus, and quantification method
First Claim
1. A biosensor for quantifying a substrate included in a sample liquid comprising:
- a first insulating support and a second insulating support;
an electrode part comprising at least a working electrode and a counter electrode;
a specimen supply path for introducing the sample liquid to the electrode part; and
a reagent layer employed for quantifying the substrate included in the sample liquid,where the electrode part, the specimen supply path, and the reagent layer are situated between the first insulating support and the second insulating support,the specimen supply path being provided on the electrode part, and the reagent layer being provided on the electrode part in the specimen supply path, respectively,the electrode part being dividedly formed by a first type of slits provided on an electrical conductive layer which is formed on the whole or part of an internal surface of one or both of the first insulating support and the second insulating support,the reagent layer is formed by dripping a reagent, anda second type of slits is provided around a position where the reagent is dripped on the electrode part so as to partly surround the dripped position and be in contact with the reagent, said second type of slits restricting the spread of the reagent.
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Accused Products
Abstract
A biosensor is disclosed comprising a support; a conductive layer composed of an electrical conductive material such as a noble metal, for example gold or palladium, and carbon; slits parallel to and perpendicular to the side of the support; working, counter, and detecting electrodes; a spacer which covers the working, counter, and detecting electrodes on the support; a rectangular cutout in the spacer forming a specimen supply path; an inlet to the specimen supply path; a reagent layer formed by applying a reagent containing an enzyme to the working, counter, and detecting electrodes, which are exposed through the cutout in the spacer; and a cover over the spacer. The biosensor can be formed by a simple method, and provides a uniform reagent layer on the electrodes regardless of the reagent composition.
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Citations
21 Claims
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1. A biosensor for quantifying a substrate included in a sample liquid comprising:
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a first insulating support and a second insulating support; an electrode part comprising at least a working electrode and a counter electrode; a specimen supply path for introducing the sample liquid to the electrode part; and a reagent layer employed for quantifying the substrate included in the sample liquid, where the electrode part, the specimen supply path, and the reagent layer are situated between the first insulating support and the second insulating support, the specimen supply path being provided on the electrode part, and the reagent layer being provided on the electrode part in the specimen supply path, respectively, the electrode part being dividedly formed by a first type of slits provided on an electrical conductive layer which is formed on the whole or part of an internal surface of one or both of the first insulating support and the second insulating support, the reagent layer is formed by dripping a reagent, and a second type of slits is provided around a position where the reagent is dripped on the electrode part so as to partly surround the dripped position and be in contact with the reagent, said second type of slits restricting the spread of the reagent. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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Specification