Conditioned cell culture medium compositions and methods of use
First Claim
1. A composition for topical administration to a subject in need thereof comprising (1) conditioned cell culture medium, said medium comprising medium free of eukaryotic cells which has previously supported the growth of eukaryotic cells cultured in three-dimensions;
- wherein the cells cultured in three-dimensions comprise stromal cells and form a three-dimensional stromal tissue; and
wherein the stromal cells comprise human fibroblast cells; and
(2) a pharmaceutically or cosmetically acceptable carrier, wherein the composition is stable for up to 2 years in storage at −
20°
C.
3 Assignments
0 Petitions
Accused Products
Abstract
Novel products comprising conditioned cell culture medium compositions and methods of use are described. The conditioned cell medium compositions of the invention may be comprised of any known defined or undefined medium and may be conditioned using any eukaryotic cell type. The medium may be conditioned by stromal cells, parenchymal cells, mesenchymal stem cells, liver reserve cells, neural stem cells, pancreatic stem cells and/or embryonic stem cells. Additionally, the cells may be genetically modified. A three-dimensional tissue construct is preferred. Once the cell medium of the invention is conditioned, it may be used in any state. Physical embodiments of the conditioned medium include, but are not limited to, liquid or solid, frozen, lyophilized or dried into a powder. Additionally, the medium is formulated with a pharmaceutically acceptable carrier as a vehicle for internal administration, applied directly to a food item or product, formulated with a salve or ointment for topical applications, or, for example, made into or added to surgical glue to accelerate healing of sutures following invasive procedures. Also, the medium may be further processed to concentrate or reduce one or more factors or components contained within the medium.
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Citations
10 Claims
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1. A composition for topical administration to a subject in need thereof comprising (1) conditioned cell culture medium, said medium comprising medium free of eukaryotic cells which has previously supported the growth of eukaryotic cells cultured in three-dimensions;
- wherein the cells cultured in three-dimensions comprise stromal cells and form a three-dimensional stromal tissue; and
wherein the stromal cells comprise human fibroblast cells; and
(2) a pharmaceutically or cosmetically acceptable carrier, wherein the composition is stable for up to 2 years in storage at −
20°
C. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
- wherein the cells cultured in three-dimensions comprise stromal cells and form a three-dimensional stromal tissue; and
Specification