Xylose utilizing zymomonas mobilis with improved ethanol production in biomass hydrolysate medium
First Claim
Patent Images
1. A recombinant, xylose-utilizing, ethanol-producing microorganism of the genus Zymomonas, having at least one genetic modification in the zmo1432 open reading frame, wherein said zmo1432 open reading frame having at least one genetic modification encodes a polypeptide having an amino acid sequence with at least about 95% identity to the amino acid sequence as set forth in SEQ ID NO:
- 2, wherein the presence of the mutant protein increases ethanol production by the Zymomonas during fermentation in biomass hydrolysate medium as compared to production by a Zymomonas lacking the genetic modification under the same conditions.
6 Assignments
0 Petitions
Accused Products
Abstract
Xylose-utilizing, ethanol producing strains of Zymomonas mobilis with improved performance in medium comprising biomass hydrolysate were isolated using an adaptation process. Independently isolated strains were found to have independent mutations in the same coding region. Mutation in this coding may be engineered to confer the improved phenotype.
-
Citations
6 Claims
-
1. A recombinant, xylose-utilizing, ethanol-producing microorganism of the genus Zymomonas, having at least one genetic modification in the zmo1432 open reading frame, wherein said zmo1432 open reading frame having at least one genetic modification encodes a polypeptide having an amino acid sequence with at least about 95% identity to the amino acid sequence as set forth in SEQ ID NO:
- 2, wherein the presence of the mutant protein increases ethanol production by the Zymomonas during fermentation in biomass hydrolysate medium as compared to production by a Zymomonas lacking the genetic modification under the same conditions.
- View Dependent Claims (2, 3, 6)
-
4. A polynucleotide encoding a protein having the amino acid sequence selected from the group consisting of SEQ ID NO:
- 3 and SEQ ID NO;
4.
- 3 and SEQ ID NO;
-
5. A method for the production of a recombinant Zymonomas ethanologen comprising:
-
a) providing a xylose-utilizing, ethanol-producing microorganism of the genus Zymomonas comprising a zmo1432 open reading frame encoding a polypeptide having the amino acid sequence as set forth in SEQ ID NO;
2; andb) introducing a mutation in the zmo1432 open reading frame of a) such that expression of the mutated open reading frame expresses a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NO;
3 and SEQ ID NO;
4.
-
Specification