Expression of hexose kinase in recombinant host cells
First Claim
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1. A recombinant yeast host cell comprising:
- (a) a modification in an endogenous polynucleotide encoding a polypeptide having dual-role hexokinase activity in the host cell wherein the activity of the polypeptide of (a) is reduced or substantially eliminated; and
(b) a heterologous polynucleotide encoding a polypeptide having hexose kinase activity; and
(c) a modification in an endogenous polynucleotide encoding a polypeptide having pyruvate decarboxylase activity wherein pyruvate decarboxylase activity is reduced or substantially eliminated.
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Abstract
The invention relates to a recombinant host cell having (a) a modification in an endogenous polynucleotide encoding a polypeptide having dual-role hexokinase activity; (b) a heterologous polynucleotide encoding a polypeptide having hexose kinase activity; and optionally (c) a modification in an endogenous polynucleotide encoding a polypeptide having pyruvate decarboxylase activity. Additionally, the invention relates to methods of making and using such recombinant host cells including, for example, methods of increasing glucose consumption, methods of improving redox balance, and/or methods of increasing the production of a product of a pyruvate-utilizing pathway.
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Citations
30 Claims
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1. A recombinant yeast host cell comprising:
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(a) a modification in an endogenous polynucleotide encoding a polypeptide having dual-role hexokinase activity in the host cell wherein the activity of the polypeptide of (a) is reduced or substantially eliminated; and (b) a heterologous polynucleotide encoding a polypeptide having hexose kinase activity; and (c) a modification in an endogenous polynucleotide encoding a polypeptide having pyruvate decarboxylase activity wherein pyruvate decarboxylase activity is reduced or substantially eliminated. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30)
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Specification