Application of 2, 3-dihydro-3-hydroxymethyl-6-amino-[1,4]-benzoxazine in preparation of drugs inducing embryonic stem cells to differentiate to vascular endothelial cells

• US 9,101,634 B2
• Filed: 05/10/2011
• Issued: 08/11/2015
• Est. Priority Date: 05/20/2010
• Status: Expired due to Fees

• Current Assignee: Shandong University
• Original Assignee: Shandong University
• Inventors: Miao, Junying, Zhao, Baoxiang, Zhang, Shangli, Zhao, Jing, Su, Le, Han, Lei
• Primary Examiner(s): Noble, Marcia S
• Assistant Examiner(s): Hsu, Chi-Feng
• Application Number:
  US13/378,451
• Publication Number:
  US 20120123116A1
• Time in Patent Office: 1,554 Days
• US Class Current: 1/1
• CPC Class Codes: A61K 31/538 ortho- or peri-condensed with carbocyclic ring systems
• CPC Class Codes: A61P 9/00 Drugs for disorders of the cardiovascular system

Abstract

An application of 2,3-dihydro-3 hydroxymethyl-6-amino-[1,4]-benzoxazine in the preparation of drugs inducing embryonic stem cells to differentiate to vascular endothelial cells, wherein the concentration of the 2,3-dihydro-3 hydroxymethyl-6-amino-[1,4]-benzoxazine in the preparation of drugs effectively inducing mouse embryonic stem cells to differentiate to vascular endothelial cells is 1˜20 μM. The 2,3-dihydro-3 hydroxymethyl-6-amino-[1,4]-benzoxazine provided by the invention lays foundation for preparing and developing drugs inducing embryonic stem cells to differentiate to vascular endothelial cells, can be used as an effective research tool of embryonic stem cells, and can be used for researching on embryonic stem cell differentiation and new molecular mechanisms of blood vessel formation.

Claims

1. A method of inducing embryonic stem cells to differentiate to functional vascular endothelial cells, comprising culturing the embryonic stem cells with a culture medium comprising 2,3-dihydro-3-hydroxymethyl-6-amino-[1,4]-benzoxazine at a concentration of from 1 to 20 μ

M.

2. The method of claim 1, wherein the concentration of 2,3-dihydro-3-hydroxymethyl-6-amino-[1,4]-benzoxazine is 10 μ

M.

3. The method of claim 1, wherein the embryonic stem cells are mouse embryonic stem cells.

4. The method of claim 2, wherein the embryonic stem cells are mouse embryonic stem cells.