Cell culture vessel and method of production thereof
First Claim
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1. A method of culturing cells comprising:
- (a) disposing carbon nanotubes in a dispersion medium to form a mixture thereof, wherein the dispersion medium is a solvent primarily comprised of one of water and a lower alcohol having 1-4 carbons and wherein the carbon nanotubes have a concentration in the mixture of less than or equal to 20 mg/L,(b) subjecting the mixture to ultrasonic vibrations to form a dispersion, in which the carbon nanotubes are in a highly-dispersed state with no visible agglomerates,(c) applying an amount of the dispersion in the highly-dispersed state directly to a surface of a vessel such that a resulting carbon nanotube thin-film after drying will contain carbon nanotube material in an amount of at least 0.001 μ
g and not more than 0.1 μ
g per cm2,(d) drying the applied dispersion on the surface to form the carbon nanotube thin-film,(e) repeating steps (c) and (d) at least 10 times to form a built-up carbon nanotube layer structure having at least 10 layers of carbon nanotube thin-films and such that the built-up carbon nanotube layer structure contains the carbon nanotube material in an amount of at least 0.02 μ
g and not more than 10 μ
g per cm2,(f) sterilizing the vessel having the built-up carbon nanotube layer structure,(g) disposing cells in the sterilized vessel so that the cells directly contact the built-up carbon nanotube layer structure, and(h) subjecting the vessel having the cells disposed therein to cell culturing conditions;
wherein steps (c) and (d) are carried out in an environment having a relative humidity of less than or equal to 30% and the dispersion contains substantially no polymer ingredients.
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Accused Products
Abstract
Disclosed is a method of producing a cell culture vessel (10) having a carbon nanotube (CNT) layer (14) on its surface. The method comprises the steps of providing a vessel (12) having a predetermined shape; providing a CNT dispersion of a CNT material composed primarily of CNT dispersed in a dispersion medium at a concentration of not more than 50 mg/L; and forming the carbon nanotube layer (14) on the surface of the vessel (12). The formation of the CNT layer (14) is achieved by alternately repeating a supply step of applying the CNT dispersion solution to the vessel (12) and a drying step of drying the applied dispersion solution one or more times.
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Citations
3 Claims
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1. A method of culturing cells comprising:
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(a) disposing carbon nanotubes in a dispersion medium to form a mixture thereof, wherein the dispersion medium is a solvent primarily comprised of one of water and a lower alcohol having 1-4 carbons and wherein the carbon nanotubes have a concentration in the mixture of less than or equal to 20 mg/L, (b) subjecting the mixture to ultrasonic vibrations to form a dispersion, in which the carbon nanotubes are in a highly-dispersed state with no visible agglomerates, (c) applying an amount of the dispersion in the highly-dispersed state directly to a surface of a vessel such that a resulting carbon nanotube thin-film after drying will contain carbon nanotube material in an amount of at least 0.001 μ
g and not more than 0.1 μ
g per cm2,(d) drying the applied dispersion on the surface to form the carbon nanotube thin-film, (e) repeating steps (c) and (d) at least 10 times to form a built-up carbon nanotube layer structure having at least 10 layers of carbon nanotube thin-films and such that the built-up carbon nanotube layer structure contains the carbon nanotube material in an amount of at least 0.02 μ
g and not more than 10 μ
g per cm2,(f) sterilizing the vessel having the built-up carbon nanotube layer structure, (g) disposing cells in the sterilized vessel so that the cells directly contact the built-up carbon nanotube layer structure, and (h) subjecting the vessel having the cells disposed therein to cell culturing conditions; wherein steps (c) and (d) are carried out in an environment having a relative humidity of less than or equal to 30% and the dispersion contains substantially no polymer ingredients.
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2. A method of culturing cells comprising:
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(a) disposing carbon nanotubes in a dispersion medium to form a mixture thereof, wherein the dispersion medium is a solvent primarily comprised of one of water and a lower alcohol having 1-4 carbons and wherein the carbon nanotubes have a concentration in the mixture of less than or equal to 20 mg/L, (b) subjecting the mixture to ultrasonic vibrations to form a dispersion, in which the carbon nanotubes are in a highly-dispersed state with no visible agglomerates, (c) applying an amount of the dispersion in the highly-dispersed state directly to a surface of a vessel such that a resulting carbon nanotube thin-film after drying will contain carbon nanotube material in an amount of at least 0.001 μ
g and not more than 0.1 μ
g per cm2,(d) drying the applied dispersion on the surface to form the carbon nanotube thin-film, (e) repeating steps (c) and (d) at least 10 times to form a built-up carbon nanotube layer structure having at least 10 layers of carbon nanotube thin-films and such that the built-up carbon nanotube layer structure contains the carbon nanotube material in an amount of at least 0.02 μ
g and not more than 10 μ
g per cm2,(f) sterilizing the vessel having the built-up carbon nanotube layer structure, (g) disposing cells in the sterilized vessel so that the cells directly contact the built-up carbon nanotube layer structure, and (h) subjecting the vessel having the cells disposed therein to cell culturing conditions; wherein steps (c) and (d) are carried out at room temperature and atmospheric pressure and the dispersion contains substantially no polymer ingredients.
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3. A method of culturing cells comprising:
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(a) disposing carbon nanotubes in a dispersion medium to form a mixture thereof, wherein the dispersion medium is a solvent primarily comprised of one of water and a lower alcohol having 1-4 carbons and wherein the carbon nanotubes have a concentration in the mixture of less than or equal to 20 mg/L, (b) subjecting the mixture to ultrasonic vibrations to form a dispersion, in which the carbon nanotubes are in a highly-dispersed state with no visible agglomerates, (c) applying an amount of the dispersion in the highly-dispersed state directly to a surface of a vessel such that a resulting carbon nanotube thin-film after drying will contain carbon nanotube material in an amount of at least 0.001 μ
g and not more than 0.1 μ
g per cm2,(d) drying the applied dispersion on the surface to form the carbon nanotube thin-film, (e) repeating steps (c) and (d) at least 10 times to form a built-up carbon nanotube layer structure having at least 10 layers of carbon nanotube thin-films and such that the built-up carbon nanotube layer structure contains the carbon nanotube material in an amount of at least 0.02 μ
g and not more than 10 μ
g per cm2,(f) sterilizing the vessel having the built-up carbon nanotube layer structure, (g) disposing cells in the sterilized vessel so that the cells directly contact the built-up carbon nanotube layer structure, and (h) subjecting the vessel having the cells disposed therein to cell culturing conditions; wherein steps (c) and (d) are repeated less than or equal to 200 times or between 20-50 times, wherein the dispersion comprises volatile and non-volatile ingredients and the carbon nanotubes account for at least 95 wt % of the non-volatile ingredients in the dispersion, wherein steps (c) and (d) are carried out in an environment having a relative humidity of less than or equal to 30% and at room temperature and atmospheric pressure; and
the dispersion contains substantially no polymer ingredients.
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Specification