Methods for the production of IPS cells using Epstein-Barr (EBV)-based reprogramming vectors
First Claim
1. An isolated population of human induced pluripotent stem (iPS) cells, said population comprising human pluripotent cells that comprise Epstein-Barr virus (EBV)-based reprogramming vectors comprising a replication origin and one or more expression cassettes encoding iPS reprogramming factors comprising at least Sox-2, Oct-4, wherein said cells, or one or more of said expression cassettes, comprise a nucleotide sequence encoding a trans-acting factor that binds to the replication origin to replicate an extra-chromosomal template, the cell population comprising the genome of a selected adult human individual and essentially free of chromosomally integrated retroviral elemants.
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Abstract
Methods and composition of induction of pluripotent stem cells and other desired cell types are disclosed. For example, in certain aspects methods for generating essentially vector-free induced pluripotent stem cells are described. Furthermore, the invention provides induced pluripotent stem cells and desired cell types essentially free of exogenous vector elements with the episomal expression vectors to express differentiation programming factors.
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Citations
10 Claims
- 1. An isolated population of human induced pluripotent stem (iPS) cells, said population comprising human pluripotent cells that comprise Epstein-Barr virus (EBV)-based reprogramming vectors comprising a replication origin and one or more expression cassettes encoding iPS reprogramming factors comprising at least Sox-2, Oct-4, wherein said cells, or one or more of said expression cassettes, comprise a nucleotide sequence encoding a trans-acting factor that binds to the replication origin to replicate an extra-chromosomal template, the cell population comprising the genome of a selected adult human individual and essentially free of chromosomally integrated retroviral elemants.
- 5. An isolated population of human induced pluripotent stem (iPS) cells, said population comprising human pluripotent cells that comprise Epstein-Barr virus (EBV)-based reprogramming vectors comprising a replication origin and one or more expression cassettes encoding at least the reprogramming factors Oct4 and Sox2, and additionally one or more of the reprogramming factors selected from the group consisting of Lin28, Nanog, Klf4 and c-Myc, wherein said cells, or one or more of said expression cassettes, comprise a nucleotide sequence encoding a trans-acting factor that binds to the replication origin to replicate an extra-chromosomal template, wherein the iPS cells of the population have been produced from somatic cells of a selected human individual and comprise the genome of that individual and are essentially free of chromosomally integrated retroviral elements.
Specification