Methods and apparatus for measuring analytes using large scale FET arrays

• US 10,379,079 B2
• Filed: 12/16/2015
• Issued: 08/13/2019
• Est. Priority Date: 12/18/2014
• Status: Active Grant

• Current Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Original Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Inventors: Fife, Keith G.
• Primary Examiner(s): Kessel, Maris R
• Assistant Examiner(s): Allen, Joshua L
• Application Number:
  US14/971,435
• Publication Number:
  US 20160178570A1
• Time in Patent Office: 1,336 Days
• Field of Search: 257253
• CPC Class Codes: G01N 27/414 Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
• CPC Class Codes: G01N 27/4148 Integrated circuits therefor, e.g. fabricated by CMOS processing CMOS processing per se H01L21/82

Abstract

A semiconductor device, comprising a first field effect transistor (FET) connected in series to a second FET, and a third FET connected in series to the first FET and the second FET. The semiconductor device further includes bias circuitry coupled to the first FET and the second FET, and an output conductor coupled to a terminal of the second FET, wherein the output conductor obtains an output signal from the second FET that is independent of the first FET.

Claims

1. A semiconductor device, comprising:

a first field effect transistor (FET), the first FET having a first electrode proximate to a first reaction site;

a second FET connected in series to the first FET, the second FET having a second electrode proximate to a second reaction site;

a select transistor connected in series to the first FET and the second FET, wherein the select transistor concurrently couples the first FET to a first output conductor and the second FET to a second output conductor in response to a select signal; and

bias circuitry coupled to the first FET and the second FET, wherein the bias circuitry is configured to apply a voltage to one of either the first FET or the second FET, and to concurrently apply a current sink to the other of the first or the second FET.

2. The device of claim 1, wherein an output signal from the other of the first FET obtained on the first output conductor or the second FET obtained on the second output conductor is independent of the voltage applied to one of either the first FET or the second FET.

3. The device of claim 1, wherein the bias circuitry comprises at least one voltage source and at least one current sink.

4. The device of claim 1, wherein the bias circuitry is configured to operate one of either the first FET or the second FET under at least one of drain induced barrier lowering or punch through mode constraints.

5. The device of claim 4, wherein when one of either the first FET or the second FET operates under drain induced barrier lowering or punch through mode constraints, an output signal is obtained from the other of the first FET or the second FET.

6. The device of claim 1, wherein the select transistor is biased in at least one of a triode region and a saturation region.

7. The device of claim 1, wherein the first FET and the second FET are chemically-sensitive field effect transistors (chemFETs).

8. The device of claim 1, wherein the first FET and the second FET are ion-sensitive field effect transistors (ISFETs).

9. The device of claim 8, wherein the ISFETS are sensitive to hydrogen ions.

10. The device of claim 1, wherein the select transistor is a FET.

11. The device of claim 1, wherein the first electrode proximate to the first reaction site is coupled to a floating gate structure.

12. The device of claim 1, wherein the second electrode proximate to the second reaction site is coupled to a floating gate structure.