Intracellular delivery of biological effectors
First Claim
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1. A transporter peptide comprising at least one amino acid sequence selected from the group consisting of:
- a) (XmRXoRXn);
b) (XmRRRXn);
c) (XmRRXRXn); and
d) (XmRXRRXn),
wherein X is a non-basic amino acid;
m is an integer from zero to fourteen;
n is an integer, independent of in, between zero and fourteen;
o is an integer, independent of m and n between zero and five; and
wherein said transporter peptide is capable of translocating across a biological membrane.
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Abstract
The invention relates to a sequence of amino acids with the capacity to facilitate transport of an effector across a biological membrane. More specifically, the present invention relates to novel peptide transporters that specifically target certain cell types for the intracellular delivery of drugs and therapeutic agents.
87 Citations
35 Claims
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1. A transporter peptide comprising at least one amino acid sequence selected from the group consisting of:
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a) (XmRXoRXn);
b) (XmRRRXn);
c) (XmRRXRXn); and
d) (XmRXRRXn),
whereinX is a non-basic amino acid;
m is an integer from zero to fourteen;
n is an integer, independent of in, between zero and fourteen;
o is an integer, independent of m and n between zero and five; and
wherein said transporter peptide is capable of translocating across a biological membrane. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 24, 25, 26, 27, 28, 29, 30)
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23. A method of translocating an effector into the cytoplasm and nucleus of a eukaryotic cell, said method comprising:
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a) conjugating said effector to the transporter peptide of claim I to form a transporter peptide-effector conjugate; and
b) introducing said transporter peptide-effector conjugate to the cell.
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31. A method of screening a phage library for transporter peptides, said method comprising:
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a) providing a phage display library;
b) screening said library against specific cell types; and
c) determining the cells having internalized phages. - View Dependent Claims (32, 33, 34)
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35. A transporter peptide, wherein the amino acid sequence is selected from the group consisting of SEQ ID NOS:
- 1-34.
Specification