Intracellular delivery of biological effectors

• US 6,960,648 B2
• Filed: 10/15/2001
• Issued: 11/01/2005
• Est. Priority Date: 10/13/2000
• Status: Expired due to Fees

• Current Assignee: Xigen SA
• Original Assignee: Université de Lausanne
• Inventors: Bonny, Christophe
• Primary Examiner(s): Ponnaluri, Padmashri
• Application Number:
  US09/977,831
• Publication Number:
  US 20020120100A1
• Time in Patent Office: 1,478 Days
• Field of Search: 435/6, 435/325, 435/DIG.35, 530/330, 424/192.1, 424/193.1, 424/9.1
• US Class Current: 530/330
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61K 47/62 the modifying agent being a protein, peptide or polyamino acid
• CPC Class Codes: A61K 47/65 Peptidic linkers, binders or spacers, e.g. peptidic enzyme-labile linkers
• CPC Class Codes: A61P 11/00 Drugs for disorders of the respiratory system
• CPC Class Codes: A61P 25/00 Drugs for disorders of the nervous system
• CPC Class Codes: A61P 25/28 for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
• CPC Class Codes: A61P 3/10 for hyperglycaemia, e.g. antidiabetics
• CPC Class Codes: A61P 31/12 Antivirals
• CPC Class Codes: A61P 35/00 Antineoplastic agents
• CPC Class Codes: A61P 5/48 of the pancreatic hormones
• CPC Class Codes: A61P 9/00 Drugs for disorders of the cardiovascular system
• CPC Class Codes: A61P 9/04 Inotropic agents, i.e. stimulants of cardiac contraction; Drugs for heart failure
• CPC Class Codes: A61P 9/10 for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
• CPC Class Codes: C07K 1/047 Simultaneous synthesis of different peptide species; Peptide libraries
• CPC Class Codes: C07K 2319/00 Fusion polypeptide
• CPC Class Codes: C07K 5/1013 the side chain containing O or S as heteroatoms, e.g. Cys, Ser
• CPC Class Codes: C07K 5/1016 and aromatic or cycloaliphatic
• CPC Class Codes: C07K 5/1019 with the first amino acid being basic
• CPC Class Codes: C07K 5/1021 with the first amino acid being acidic
• CPC Class Codes: C07K 7/06 having 5 to 11 amino acids

Abstract

The invention relates to a sequence of amino acids with the capacity to facilitate transport of an effector across a biological membrane. More specifically, the present invention relates to novel peptide transporters that specifically target certain cell types for the intracellular delivery of drugs and therapeutic agents.

Claims

1. A method of translocating a transporter peptide into a pancreatic B-cell, comprising contacting a pancreatic B-cell with a transporter peptide for a time and under conditions sufficient to allow a transporter peptide to translocate across a membrane of the B-cell, wherein the transporter peptide is SEQ ID NO:

1.

2. The method of claim 1, wherein the transporter peptide contains one or more modified residues.

3. The method of claim 1, wherein the pancreatic B-cell is contacted in vivo.

4. The method of claim 1, wherein the pancreatic B-cell is contacted with a pharmaceutically acceptable salt of the transporter peptide.