Sensitive coded detection systems
First Claim
1. A method of detecting analytes, the method comprising associating one or more detectors with one or more target samples, wherein the detectors each comprise a specific binding molecule, a carrier, and a block group, wherein the block group comprises blocks, and detecting the block group.
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Accused Products
Abstract
Disclosed are compositions and methods for sensitive multiplex detection of analytes. The disclosed compositions, referred to as detectors, accomplish this detection by associating specific binding molecules—which interact with desired targets—with block groups in a carrier. The block groups are made up of blocks which, through the combination of different blocks, constitute a code for a given detector. The blocks are detectable and each detector is distinguishable from other detectors by its block group. The coding of the block groups greatly increasing the number of distinguishable detectors from a relatively small number of blocks. The detection burden remains low even with such a large number of block groups because only the blocks need be distinguished from each other during detection. Numerous block molecules of each type making up the block group can be present in the carrier to effectively amplify the signal generated from targets.
96 Citations
116 Claims
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1. A method of detecting analytes, the method comprising
associating one or more detectors with one or more target samples, wherein the detectors each comprise a specific binding molecule, a carrier, and a block group, wherein the block group comprises blocks, and detecting the block group.
- 48. A composition for detecting an analyte comprising a specific binding molecule, a carrier, and a block group.
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82. A set of detectors comprising a plurality of detectors,
wherein each detectors comprises a specific binding molecule, a carrier, and a block group, wherein the block group comprises blocks, wherein each block group has a different composition of blocks.
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110. A set of block groups comprising a plurality of block groups,
wherein each block group comprises blocks, wherein the blocks comprise reporter signals, wherein the reporter signals have a common property, wherein the common property allows the reporter signals to be distinguished or separated from molecules lacking the common property, wherein the reporter signals can be altered, wherein the altered forms of each reporter signal can be distinguished from every other altered form of reporter signal.
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111. A set of blocks comprising a plurality of blocks,
wherein the blocks comprise reporter signals, wherein the reporter signals have a common property, wherein the common property allows the reporter signals to be distinguished or separated from molecules lacking the common property, wherein the reporter signals can be altered, wherein the altered forms of each reporter signal can be distinguished from every other altered form of reporter signal.
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112. A kit comprising a set of detectors, wherein the set of detectors comprises a plurality of detectors,
wherein each detectors comprises a specific binding molecule, a carrier, and a block group, wherein the block group comprises blocks.
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114. A mixture comprising
a set of detectors and a target sample, wherein the set of detectors comprises a plurality of detectors, wherein each detectors comprises a specific binding molecule, a carrier, and a block group, wherein the block group comprises blocks.
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116. A method of detecting analytes, the method comprising
associating one or more detectors with one or more target samples, wherein the detectors each comprise a specific binding molecule, a carrier, and a block group, and detecting the block group, wherein the block group comprises blocks, wherein the blocks comprise reporter signals, wherein the reporter signals have a common property, wherein the common property allows the reporter signals to be distinguished or separated from molecules lacking the common property, wherein the reporter signals can be altered, wherein the altered forms of each reporter signal can be distinguished from every other altered form of reporter signal, wherein the common property is mass-to-charge ratio, wherein the reporter signals are altered by altering their mass, wherein the altered forms of the reporter signals can be distinguished via differences in the mass-to-charge ratio of the altered forms of reporter signals, wherein the mass of the reporter signals is altered by fragmentation, wherein the block group comprises ten or more different reporter signals, wherein the reporter signals comprise peptides, wherein the peptides have the same mass-to-charge ratio, wherein the peptides have the same amino acid composition, wherein the peptides have the same amino acid sequence, wherein each peptide contains a different distribution of heavy isotopes.
Specification