Sensor for detecting biomolecule using carbon nanotubes

• US 20030134267A1
• Filed: 09/27/2002
• Published: 07/17/2003
• Est. Priority Date: 08/14/2001
• Status: Abandoned Application

• Current Assignee: Samsung Electronics Co. Ltd.
• Original Assignee: Samsung Electronics Co. Ltd.
• Inventors: Kang, Seong-Ho, Choi, Won-Bong, Pak, Yukeun Eugene
• Application Number:
  US10/240,227
• Publication Number:
  US 20030134267A1
• Time in Patent Office: Days
• US Class Current: 435/4
• CPC Class Codes: G01N 33/5438 Electrodes

Abstract

Provided is a sensor for detecting a biomolecule, and particularly, a sensor for detecting a biomolecule, including (a) a substrate; and (b) a plurality of carbon nanotubes which are arranged on the substrate and provide a binding site for a receptor for a target biomolecule. With the biomolecular sensor, a various kinds of disease-associated biomolecules can be detected simultaneously, accurately and quickly.

Claims

1. A sensor for detecting a biomolecule comprising:

(a) a substrate; and

(b) a plurality of carbon nanotubes which are arranged on the substrate, and provide a binding site for a receptor for a target biomolecule.

2. The sensor for detecting a biomolecule of claim 1, wherein the substrate is formed of a material selected from the group consisting of silicon, glass, molten silica, plastics, and polydimethylsiloxane (PDMS).

3. The sensor for detecting a biomolecule of claim 1, wherein the receptors are selected from the group consisting of nucleic acids, proteins, peptides, amino acids, ligands, enzyme substrates, cofactors, and oligosaccharides.

4. The sensor for detecting a biomolecule of claim 1, wherein the target biomolecule is selected from the group consisting of proteins, nucleic acids and enzymes.

5. The sensor for detecting a biomolecule of claim 1, further comprising a detection system for detecting the immobilization of the receptors on the carbon nanotubes or the binding of the biomolecules to the receptors.

6. The sensor for detecting a biomolecule of claim 5, wherein the detection system comprises an electrical detector or an x-y fluorescent laser reader.

7. A sensor for detecting a biomolecule comprising:

(a) a substrate;

(b) micro- or nano-sized multiple channels formed in the substrate; and

(c) one or more carbon nanotubes which are arranged at a particular position within the multiple channels, and provide a binding site for a receptor for a target biomolecule.

8. The sensor for detecting a biomolecule of claim 7, wherein the substrate is formed of a material selected from the group consisting of silicon, glass, molten silica, plastics, and polydimethylsiloxane (PDMS).

9. The sensor for detecting a biomolecules of claim 7, wherein the receptors are selected from the group consisting of nucleic acids, proteins, peptides, amino acids, ligands, enzyme substrates, cofactors, and oligosaccharides.

11. The sensor for detecting a biomolecule of claim 7, further comprising a detection system for detecting the immobilization of the receptors on the carbon nanotubes or the binding of the biomolecules to the receptors.

12. The sensor for detecting a biomolecule of claim 11, the detection system comprises an electrical detector or an x-y fluorescent laser reader.

13. The sensor for detecting a biomolecule of claim 7, wherein each of the multiple channels further comprises a delivery and separation system to deliver and separate the biomolecules to be assayed according to their size and electrical properties.

14. The sensor for detecting a biomolecule of claim 13, wherein the delivery and separation system comprises a micro-pump or a capillary electrophoretic device.

10. The sensor for detecting a biomolecule of 7, wherein a target biomolecule is selected from the group consisting of proteins, nucleic acids and enzymes.

15. A method for manufacturing a sensor for detecting a biomolecule comprising:

forming a plurality of cavities of a diameter of several to hundreds of nanometers at an interval of several to hundreds of nanometers in a dielectric layer; and

vertically growing a plurality of carbon nanotubes through the plurality of cavities.

16. A method for detecting a biomolecule comprising:

forming a plurality of carbon nanotubes on a substrate;

applying a constant or different levels of an electric field of a polarity opposite to a net charge of each of receptors, to the carbon nanotubes in order to move and bind a receptor that specifically interacts with a target biomolecule to a corresponding carbon nanotubes;

applying a sample to the receptor bound carbon nanotubes; and

detecting the target biomolecule bound to the receptor to determine the target biomolecule.

17. The method of claim 16, further comprising, before or after immobilizing the receptors on the corresponding carbon nanotube, treating an auxiliary binder to enhance an adhesion between the carbon nanotubes and the receptors.

18. The method of claim 17, wherein the auxiliary binder is selected from the group consisting of a chemical having an aldehyde, amino, or imido group at its carbonyl end, a SiO₂ monolayer, a Si₃N₄ monolayer, a nitrocellulose membrane, polyacrylamide gel and polydimethylsiloxane (PDMS).

19. The method of claim 16, wherein different kinds of target biomolecules are simultaneously analyzed on a mass scale by directly detecting the target biomolecules bound to various kinds of receptors or by measuring a difference in binding force of the target molecules to the receptors.

20. The method of claim 16, wherein the target biomolecule is a disease-associated target protein.