Device and method for collecting, transporting and recovering low molecular weight analytes in saliva
First Claim
1. A device for sequestering a low molecular weight analyte from a saliva sample and storing the low molecular weight analyte until detection, comprising:
- (a) a matrix, said matrix being able to absorb water from the sample and reversibly adsorb the low molecular weight analyte from the sample, and said matrix comprising cavities sized to accommodate the low molecular weight analyte within, and to exclude selected components of saliva, and (b) a container, into which the saliva sample and the matrix may be placed.
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Accused Products
Abstract
This invention relates to low molecular weight analyte sequestration, storage, transport and recovery device and methods for its use with saliva samples in the detection of glucose and other low molecular weight analytes. A stimulated or non-stimulated saliva sample is contacted with a matrix (16) disposed within a container (12) that adsorbs glucose, absorbs water and excludes molecules above a certain size when hydrated. After hydration of the matrix by the saliva sample, the glucose is both adsorbed to the matrix, and migrates into the cavities within the matrix, whereas microbes such as bacteria and larger molecules such as proteins cannot. A membrane (18) keeps the matrix (16) in the bottom of the container (12). The sample is then transported to a location where the glucose will be eluted from the matrix and assayed.
25 Citations
58 Claims
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1. A device for sequestering a low molecular weight analyte from a saliva sample and storing the low molecular weight analyte until detection, comprising:
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(a) a matrix, said matrix being able to absorb water from the sample and reversibly adsorb the low molecular weight analyte from the sample, and said matrix comprising cavities sized to accommodate the low molecular weight analyte within, and to exclude selected components of saliva, and (b) a container, into which the saliva sample and the matrix may be placed. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 34, 35)
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17. A method of sequestering a low molecular weight analyte from a saliva sample, comprising:
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(b) providing;
(i) a matrix, said matrix being able to absorb water from the sample and reversibly adsorb the low molecular weight analyte from the sample, and said matrix comprising cavities sized to accommodate the low molecular weight analyte within, and to exclude selected components of saliva, and (ii) a saliva sample, and (b) contacting the matrix with the saliva sample.
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18. A method of sequestering a low molecular weight analyte from a saliva sample and storing the low molecular weight analyte until detection, comprising:
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(b) providing;
(i) a matrix, said matrix being able to absorb water from the sample and reversibly adsorb the low molecular weight analyte from the sample, and said matrix comprising cavities sized to accommodate the low molecular weight analyte within, and to exclude selected components of saliva, and (ii) a preservative;
(b) contacting the matrix with the sample and preservative, such that the matrix becomes hydrated by the sample. - View Dependent Claims (19, 20, 21, 22, 23, 24, 25, 28, 29, 30)
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26. A method of assaying for a low molecular weight analyte in a saliva sample comprising:
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(f) providing a matrix, said matrix being able to absorb water from the sample and reversibly adsorb the low molecular weight analyte from the sample, and said matrix comprising cavities sized to accommodate the low molecular weight analyte within, and to exclude selected components of saliva;
(g) contacting the matrix with the sample;
(h) removing liquid that is not absorbed into the matrix;
(i) releasing the low molecular weight analyte that is sequestered within the cavities of the matrix, and (j) detecting the released low molecular weight analyte. - View Dependent Claims (27)
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31. A kit for sequestering a low molecular weight analyte from a saliva sample and storing the low molecular weight analyte until detection, comprising:
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(a) a matrix, said matrix being able to absorb water from the sample and reversibly adsorb the low molecular weight analyte from the sample, and said matrix comprising cavities sized to accommodate the low molecular weight analyte within, and to exclude selected components of saliva, and (b) a container into which the matrix and the saliva sample may be placed. - View Dependent Claims (32, 33)
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36. A method of sequestering a low molecular weight analyte from a saliva sample and storing the low molecular weight analyte until detection, comprising:
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(a) providing;
(i) a first matrix, said first matrix being able to absorb water from the sample and reversibly adsorb the low molecular weight analyte from the sample, and said first matrix comprising cavities sized to accommodate the low molecular weight analyte within, and to exclude selected components of saliva;
(ii) a second matrix, and said second matrix being able to absorb water from the sample and said second matrix comprising cavities sized to accommodate the low molecular weight analyte within, and to exclude selected components of saliva, and (iii) a preservative;
(b) placing said first matrix at the bottom of a container, and layering said second matrix on the top surface of the first matrix, (c) introducing the sample into the container by applying the sample to the top surface of second matrix; and
(d) allowing the sample to hydrate the second matrix and then the first matrix. - View Dependent Claims (37, 38, 39, 40, 41, 42, 45, 46)
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43. A method of assaying for a low molecular weight analyte in a saliva sample comprising:
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(a) providing;
(i) a first matrix, said first matrix being able to absorb water from the sample and reversibly adsorb the low molecular weight analyte from the sample, and said first matrix comprising cavities sized to accommodate the low molecular weight analyte within, and to exclude selected components of saliva;
(ii) a second matrix, and said second matrix being able to absorb water from the sample and said second matrix comprising cavities sized to accommodate the low molecular weight analyte within, and to exclude selected components of saliva, (b) contacting the sample with the second matrix and then with the first matrix;
(c) removing liquid that is not absorbed into the first or the second matrix;
(d) releasing the low molecular weight analyte that is sequestered within the cavities of the first matrix, and (e) detecting the released low molecular weight analyte. - View Dependent Claims (44)
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47. A device for sequestering a low molecular weight analyte from a saliva sample and storing the low molecular weight analyte until detection, comprising:
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(a) a matrix, said matrix being able to absorb water from the sample and reversibly adsorb the low molecular weight analyte from the sample, and said matrix comprising cavities sized to accommodate the low molecular weight analyte within, and to exclude selected components of saliva, and (b) a solid support to which said matrix is affixed. - View Dependent Claims (48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58)
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Specification