Binding proteins as biosensors
First Claim
Patent Images
1. A glucose biosensor for in vivo or in vitro use comprising:
- at least one mutated binding protein and at least one reporter group attached thereto, such that said reporter group provides a detectable and reversible signal change when said mutated binding protein is exposed to varying glucose concentrations, wherein said detectable and reversible signal change is related to said varying concentrations.
1 Assignment
0 Petitions
Accused Products
Abstract
The invention is directed to compositions of mutated binding proteins containing reporter groups, analyte biosensor devices derived therefrom, and their use as analyte biosensors both in vitro and in vivo.
35 Citations
77 Claims
-
1. A glucose biosensor for in vivo or in vitro use comprising:
at least one mutated binding protein and at least one reporter group attached thereto, such that said reporter group provides a detectable and reversible signal change when said mutated binding protein is exposed to varying glucose concentrations, wherein said detectable and reversible signal change is related to said varying concentrations. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
-
22. A method for glucose detection comprising:
-
a) providing at least one mutated glucose/galactose binding protein and at least one reporter group attached thereto;
b) exposing said mutated glucose/galactose binding protein to varying glucose concentrations; and
c) detecting a detectable and reversible signal change from said reporter group wherein said detectable and reversible signal change corresponds to said varying glucose concentrations. - View Dependent Claims (23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42)
-
-
43. A composition comprising:
a mutated glucose/galactose binding protein having at least one amino acid substitution selected from the group consisting of a cysteine at position 1, a serine at position 1, a cysteine at position 11, a cysteine at position 14, a cysteine at position 19, a cysteine at position 43, a cysteine at position 74, a cysteine at position 107, a cysteine at position 110, a cysteine at position 112, a cysteine at position 113, a cysteine at position 137, a cysteine at position 149, a cysteine at position 213, a cysteine at position 216, a cysteine at position 238, a cysteine at position 287, a cysteine at position 292, a cysteine at position 236, and a cysteine at position 296. - View Dependent Claims (44, 45, 46, 47, 48, 49)
-
50. A composition comprising:
- a mutated glucose/galactose binding protein having at least one amino acid substitution selected from the group consisting of a cysteine at position 152 and a cysteine at position 182, and at least one additional insertion, deletion or substitution of an amino acid residue.
- View Dependent Claims (51, 52, 53, 54, 55, 56)
-
57. A composition comprising:
a mutated glucose/galactose binding protein having at least two amino acid substitutions selected from the group consisting of a cysteine at position 112 and a serine at position 238, a cysteine at position 149 and a serine at position 238, a cysteine at position 152 and a serine at position 213, a cysteine at position 213 and a cysteine at position 238, a cysteine at position 149 and an arginine at position 213, a cysteine at position 149 and a cysteine at position 213, a cysteine at position 149 and a threonine at position 213, a cysteine at position 149 and a leucine at position 213, a cysteine at position 149 and a tyrosine at position 213, a cysteine at position 149 and a serine at position 213, a cysteine at position 149 and an asparagine at position 223, a cysteine at position 149 and a cysteine at position 238, a cysteine at position 149 and a serine at position 256, a cysteine at position 149 and an arginine at position 256, a cysteine at position 152 and an arginine at position 213, a cysteine at position 152 and an asparagine at position 223, a cysteine at position 213 and a cysteine at position 255. - View Dependent Claims (58, 59, 60, 61, 62, 63)
-
64. A composition comprising:
a mutated glucose/galactose binding protein having at least three amino acid substitutions selected from the group consisting of a cysteine at position 149, a serine at position 213 and a serine at position 238;
a cysteine at position 149, an arginine at position 213 and a serine at position 238;
a cysteine at position 149, a cysteine at position 213 and a cysteine at position 238;
a cysteine at position 149, a serine at position 213 and an asparagine at position 223;
a cysteine at position 149, an asparagine at position 223 and an arginine at position 256;
a cysteine at position 149, an arginine at position 213 and a cysteine at position 238; and
a cysteine at position 149, a cysteine at position 213 and a serine at position 238.- View Dependent Claims (65, 66, 67, 68, 69, 70)
-
71. A composition comprising:
a mutated glucose/galactose binding protein having at least four amino acid substitutions selected from the group consisting of a serine at position 1, a cysteine at position 149, an arginine at position 213 and a serine at position 238;
a serine at position 1, a cysteine at position 149, a serine at position 213 and a serine at position 238; and
a cysteine at position 149, a cysteine at position 182, a cysteine at position 213 and a serine at position 238.- View Dependent Claims (72, 73, 74, 75, 76, 77)
Specification