Binding proteins as biosensors
First Claim
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1. A composition comprising:
- a mutated E. coli glucose/galactose binding protein having at least two amino acid substitutions in the amino acid sequence of SEQ ID NO;
1, the at least two amino acid substitutions being selected from the group consisting of a cysteine at position 112 and a serine at position 238, a cysteine at position 149 and a serine at position 238, a cysteine at position 152 and a serine at position 213, a cysteine at position 213 and a cysteine at position 238, a cysteine at position 149 and an arginine at position 213, a cysteine at position 149 and a cysteine at position 213, a cysteine at position 149 and a threonine at position 213, a cysteine at position 149 and a leucine at position 213, a cysteine at position 149 and a tyrosine at position 213, a cysteine at position 149 and a serine at position 213, a cysteine at position 149 and an asparagine at position 223, a cysteine at position 149 and a cysteine at position 238, a cysteine at position 149 and a serine at position 256, a cysteine at position 149 and an arginine at position 256, a cysteine at position 152 and an arginine at position 213, a cysteine at position 152 and an asparagine at position 223, and a cysteine at position 213 and a cysteine at position 255.
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Abstract
The invention is directed to compositions of mutated binding proteins containing reporter groups, analyte biosensor devices derived therefrom, and their use as analyte biosensors both in vitro and in vivo.
21 Citations
9 Claims
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1. A composition comprising:
a mutated E. coli glucose/galactose binding protein having at least two amino acid substitutions in the amino acid sequence of SEQ ID NO;
1, the at least two amino acid substitutions being selected from the group consisting of a cysteine at position 112 and a serine at position 238, a cysteine at position 149 and a serine at position 238, a cysteine at position 152 and a serine at position 213, a cysteine at position 213 and a cysteine at position 238, a cysteine at position 149 and an arginine at position 213, a cysteine at position 149 and a cysteine at position 213, a cysteine at position 149 and a threonine at position 213, a cysteine at position 149 and a leucine at position 213, a cysteine at position 149 and a tyrosine at position 213, a cysteine at position 149 and a serine at position 213, a cysteine at position 149 and an asparagine at position 223, a cysteine at position 149 and a cysteine at position 238, a cysteine at position 149 and a serine at position 256, a cysteine at position 149 and an arginine at position 256, a cysteine at position 152 and an arginine at position 213, a cysteine at position 152 and an asparagine at position 223, and a cysteine at position 213 and a cysteine at position 255.- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
Specification
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Current AssigneeBecton, Dickinson & Co
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Original AssigneeBecton, Dickinson & Co
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InventorsWright, David J., Hsieh, Helen Vivian, Nycz, Colleen M., Sherman, Douglas B., Pitner, J. Bruce, Amiss, Terry J.
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Primary Examiner(s)Desai; Anand U
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Application NumberUS10/776,643Publication NumberTime in Patent Office2,497 DaysField of SearchNoneUS Class Current530/350CPC Class CodesC07K 14/245 Escherichia (G)G01N 33/543 with an insoluble carrier f...G01N 33/54373 involving physiochemical en...G01N 33/5438 ElectrodesG01N 33/66 involving blood sugars, e.g...
