Electrochemical detection of nucleic acid hybridization

• US 20050233358A1
• Filed: 03/04/2005
• Published: 10/20/2005
• Est. Priority Date: 06/27/1995
• Status: Abandoned Application

• Current Assignee: Carson R. Loomis, Dean H. Johnston, Jinheung Kim, Mark F. Sistare, Mary E. Napier, Thorp H. Holden
• Original Assignee: Carson R. Loomis, Dean H. Johnston, Jinheung Kim, Mark F. Sistare, Mary E. Napier, Thorp H. Holden
• Inventors: Kim, Jinheung, Johnston, Dean H., Thorp, H. Holden, Napier, Mary E., Loomis, Carson R., Sistare, Mark F.
• Application Number:
  US11/072,388
• Publication Number:
  US 20050233358A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C12Q 1/6816 characterised by the detection means C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 1/6825 Nucleic acid detection involving sensors
• CPC Class Codes: C12Q 1/6869 Methods for sequencing
• CPC Class Codes: C12Q 2525/117 incorporating modified base
• CPC Class Codes: C12Q 2525/197 incorporating a spacer/coupling moiety
• CPC Class Codes: C12Q 2563/113 the label being electroactive, e.g. redox labels
• CPC Class Codes: C12Q 2600/156 Polymorphic or mutational markers

Abstract

A method of detecting a nucleic acid (e.g., DNA, RNA) that contains at least one preselected base (e.g., adenine, guanine, 6-mercaptoguanine, 8-oxo-guanine, and 8-oxo-adenine) comprises (a) reacting the nucleic acid with a transition metal complex capable of oxidizing the preselected base in an oxidation-reduction reaction; (b) detecting the oxidation-reduction reaction; and (c) determining the presence or absence of the nucleic acid from the detected oxidation-reduction reaction at the preselected base. The method may be used in a variety of applications, including DNA sequencing, diagnostic assays, and quantitative analysis.

Claims

1. A method of detecting DNA hybridization comprising:

(a) contacting a DNA sample with an oligonucleotide probe to form a hybridized DNA;

(b) reacting said hybridized DNA with a transition metal complex capable of oxidizing a preselected base in said oligonucleotide probe in an oxidation-reduction reaction, said oligonucleotide probe having at least one of said preselected bases;

(c) detecting said oxidation-reduction reaction;

(d) determining the presence or absence of hybridized DNA from said detected oxidation-reduction reaction at said preselected base.

2-151. -151. (canceled)