Antigen array and diagnostic uses thereof

• US 20050260770A1
• Filed: 03/31/2005
• Published: 11/24/2005
• Est. Priority Date: 04/01/2004
• Status: Abandoned Application

• Current Assignee: Yeda Research and Development Co. Ltd. (Weizmann Institute of Science)
• Original Assignee: Yeda Research and Development Co. Ltd. (Weizmann Institute of Science)
• Inventors: Quintana, Francisco Javier, Elizur, Gad, Hagedorn, Peter H., Cohen, Irun R., Domany, Eytan
• Application Number:
  US11/094,142
• Publication Number:
  US 20050260770A1
• Time in Patent Office: Days
• US Class Current: 436/518
• CPC Class Codes: G01N 33/564 for pre-existing immune complex or autoimmune disease , i.e. systemic lupus erythematosus, rheumatoid arthritis, multiple sclerosis, rheumatoid factors or complement components C1-C9

Abstract

A method of diagnosing an immune disease, or a predisposition thereto, in a subject is disclosed. The method comprises determining a capacity of immunoglobulins of the subject to specifically bind each antigen probe of an antigen probe set, wherein the antigen probe set comprises a plurality of antigen probes selected from the group consisting of at least a portion of a cell/tissue structure molecule, at least a portion of a heat shock protein, at least a portion of an immune system molecule, at least a portion of a homopolymeric polypeptide, at least a portion of a hormone, at least a portion of a metabolic enzyme, at least a portion of a microbial antigen, at least a portion of a molluscan antigen, at least a portion of a nucleic acid, at least a portion of a plant antigen, at least a portion of plasma molecule, and at least a portion of a tissue antigen, wherein the capacity is indicative of the immune disease or the predisposition thereto, thereby diagnosing the immune disease, or the predisposition thereto, in the subject.

Claims

1. A method of diagnosing an immune disease, or a predisposition thereto, in a subject, the method comprising determining a capacity of immunoglobulins of the subject to specifically bind each antigen probe of an antigen probe set, wherein said antigen probe set comprises a plurality of antigen probes selected from the group consisting of at least a portion of a cell/tissue structure molecule, at least a portion of a heat shock protein, at least a portion of an immune system molecule, at least a portion of a homopolymeric polypeptide, at least a portion of a hormone, at least a portion of a metabolic enzyme, at least a portion of a microbial antigen, at least a portion of a molluscan antigen, at least a portion of a nucleic acid, at least a portion of a plant antigen, at least a portion of plasma molecule, and at least a portion of a tissue antigen, wherein said capacity is indicative of the immune disease or the predisposition thereto, thereby diagnosing the immune disease, or the predisposition thereto, in the subject.

2. The method of claim 1, wherein said plurality of antigen probes comprises a number of distinct antigen probes selected from the group consisting of 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, and 91 antigen probes.

3. The method of claim 1, wherein said immunoglobulins belong to the IgG isotype, and whereas said plurality of antigen probes is selected from the group consisting of at least a portion of a human 70 kDa heat shock protein, at least a portion of a cardiac hormone, at least a portion of a neurotransmitter, at least a portion of a pigmentation hormone, at least a portion of a vascular hormone, at least a portion of an albumin, at least a portion of a lipoprotein, at least a portion of a nerve system molecule, and at least a portion of a pancreatic molecule.

4. The method of claim 1, wherein said immunoglobulins belong to the IgG isotype, and whereas said plurality of antigen probes is selected from the group consisting of a bacterial heat shock protein, at least a portion of a 60 kDa human heat shock protein, at least a portion of a 70 kDa human heat shock protein, at least a portion of a metabolic enzyme, at least a portion of a lipopolysaccharide, at least a portion of a molluscan antigen, at least a portion of a wheat antigen, and at least a portion of a nerve system molecule.

5. The method of claim 1, wherein said immunoglobulins belong to the IgG isotype, and whereas said plurality of antigen probes is selected from the group consisting of at least a portion of a 60 kDa human heat shock protein, at least a portion of a 70 kDa human heat shock protein, at least a portion of a cytokine receptor, at least a portion of a T-cell receptor, at least a portion of a metabolic enzyme, at least a portion of a coagulation regulator, at least a portion of a nerve system molecule, and at least a portion of a pancreatic molecule.

6. The method of claim 1, wherein said immunoglobulins belong to the IgG isotype, and whereas said plurality of antigen probes is selected from the group consisting of at least a portion of a bacterial heat shock protein, at least a portion of a human 60 kDa heat shock protein, at least a portion of a human 70 kDa heat shock protein, and at least a portion of a glutathione S-transferase.

7. The method of claim 1, wherein said immunoglobulins belong to the IgM isotype, and whereas said plurality of antigen probes is selected from the group consisting of at least a portion of a muscle structure protein, at least a portion of a 70 kDa human heat shock protein, at least a portion of a homopolymeric polypeptide, at least a portion of a hypothalamic hormone, at least a portion of a neurotransmitter, at least a portion of a matrix metalloproteinase, at least a portion of a viral antigen, at least a portion of a wheat antigen, at least a portion of an albumin, and at least a portion of a lipoprotein.

8. The method of claim 1, wherein said immunoglobulins belong to the IgM isotype, and whereas said plurality of antigen probes is selected from the group consisting of at least a portion of a crystallin, at least a portion of a bacterial heat shock protein, at least a portion of a human 60 kDa heat shock protein, at least a portion of a human 70 kDa heat shock protein, at least a portion of a complement molecule, at least a portion of a cytokine, and at least a portion of a nucleic acid.

9. The method of claim 1, wherein said immunoglobulins belong to the IgM isotype, and whereas said plurality of antigen probes is selected from the group consisting of at least a portion of a human 70 kDa heat shock protein, at least a portion of a cytokine, at least a portion of a metabolic enzyme, at least a portion of a viral antigen, at least a portion of a molluscan antigen, at least a portion of an albumin, at least a portion of a lipoprotein, at least a portion of a cartilage antigen, and at least a portion of a nerve system molecule.

10. The method of claim 1, wherein said immunoglobulins belong to the IgM isotype, and whereas said plurality of antigen probes is selected from the group consisting of at least a portion of a bacterial heat shock protein, at least a portion of a human 60 kDa heat shock protein, at least a portion of a human 70 kDa heat shock protein, at least a portion of T-cell receptor, at least a portion of a lipopolysaccharide and at least a portion of a pancreatic molecule.

11. The method of claim 1, wherein said determining said capacity of said immunoglobulins to specifically bind each antigen probe of said plurality of antigen probes comprises:

(a) exposing said immunoglobulins to said plurality of antigen probes, wherein each antigen probe of said plurality of antigen probes is attached to a distinct addressable location of a plurality of addressable locations of a support of an antigen probe array; and

(b) measuring a hybridization of said each antigen probe of said plurality of antigen probes with said immunoglobulins.

12. The method of claim 1, wherein said immune disease is an autoimmune disease.

13. The method of claim 1, wherein said immune disease is type I diabetes.

14. The method of claim 1, wherein said immune disease is a pancreatic immune disease.

15. An antigen probe array comprising:

(a) a support which comprises a plurality of addressable locations; and

(b) an antigen probe set which comprises a plurality of antigen probes selected from the group consisting of at least a portion of a cell/tissue structure molecule, at least a portion of a heat shock protein, at least a portion of an immune system molecule, at least a portion of a homopolymeric polypeptide, at least a portion of a hormone, at least a portion of a metabolic enzyme, at least a portion of a microbial antigen, at least a portion of a molluscan antigen, at least a portion of a nucleic acid, at least a portion of a plant antigen, at least a portion of plasma molecule, and at least a portion of a tissue antigen, wherein each antigen probe of said plurality of antigen probes is attached to a specific addressable location of said plurality of addressable locations.

16. The antigen probe array of claim 15, wherein said plurality of antigen probes comprises a number of distinct antigen probes selected from the group consisting of 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, and 91 antigen probes.

17. The antigen probe array of claim 15, wherein said plurality of antigen probes is selected from the group consisting of at least a portion of a human 70 kDa heat shock protein, at least a portion of a cardiac hormone, at least a portion of a neurotransmitter, at least a portion of a pigmentation hormone, at least a portion of a vascular hormone, at least a portion of an albumin, at least a portion of a lipoprotein, at least a portion of a nerve system molecule, and at least a portion of a pancreatic molecule.

18. The antigen probe array of claim 15, wherein said plurality of antigen probes is selected from the group consisting of a bacterial heat shock protein, at least a portion of a 60 kDa human heat shock protein, at least a portion of a 70 kDa human heat shock protein, at least a portion of a metabolic enzyme, at least a portion of a lipopolysaccharide, at least a portion of a molluscan antigen, at least a portion of a wheat antigen, and at least a portion of a nerve system molecule.

19. The antigen probe array of claim 15, wherein said plurality of antigen probes is selected from the group consisting of at least a portion of a 60 kDa human heat shock protein, at least a portion of a 70 kDa human heat shock protein, at least a portion of a cytokine receptor, at least a portion of a T-cell receptor, at least a portion of a metabolic enzyme, at least a portion of a coagulation regulator, at least a portion of a nerve system molecule, and at least a portion of a pancreatic molecule.

20. The antigen probe array of claim 15, wherein said plurality of antigen probes is selected from the group consisting of at least a portion of a bacterial heat shock protein, at least a portion of a human 60 kDa heat shock protein, at least a portion of a human 70 kDa heat shock protein, and at least a portion of a glutathione S-transferase.

21. The antigen probe array of claim 15, wherein said plurality of antigen probes is selected from the group consisting of at least a portion of a muscle structure protein, at least a portion of a 70 kDa human heat shock protein, at least a portion of a homopolymeric polypeptide, at least a portion of a hypothalamic hormone, at least a portion of a neurotransmitter, at least a portion of a matrix metalloproteinase, at least a portion of a viral antigen, at least a portion of a wheat antigen, at least a portion of an albumin, and at least a portion of a lipoprotein.

22. The antigen probe array of claim 15, wherein said plurality of antigen probes is selected from the group consisting of at least a portion of a crystallin, at least a portion of a bacterial heat shock protein, at least a portion of a human 60 kDa heat shock protein, at least a portion of a human 70 kDa heat shock protein, at least a portion of a complement molecule, at least a portion of a cytokine, and at least a portion of a nucleic acid.

23. The antigen probe array of claim 15, wherein said plurality of antigen probes is selected from the group consisting of at least a portion of a human 70 kDa heat shock protein, at least a portion of a cytokine, at least a portion of a metabolic enzyme, at least a portion of a viral antigen, at least a portion of a molluscan antigen, at least a portion of an albumin, at least a portion of a lipoprotein, at least a portion of a cartilage antigen, and at least a portion of a nerve system molecule.

24. The antigen probe array of claim 15, wherein said plurality of antigen probes is selected from the group consisting of at least a portion of a bacterial heat shock protein, at least a portion of a human 60 kDa heat shock protein, at least a portion of a human 70 kDa heat shock protein, at least a portion of T-cell receptor, at least a portion of a lipopolysaccharide and at least a portion of a pancreatic molecule.

25. An antigen probe set comprising a plurality of antigen probes selected from the group consisting of at least a portion of a cell/tissue structure molecule, at least a portion of a heat shock protein, at least a portion of an immune system molecule, at least a portion of a homopolymeric polypeptide, at least a portion of a hormone, at least a portion of a metabolic enzyme, at least a portion of a microbial antigen, at least a portion of a molluscan antigen, at least a portion of a nucleic acid, at least a portion of a plant antigen, at least a portion of plasma molecule, and at least a portion of a tissue antigen.

26. The antigen probe set of claim 25, wherein said plurality of antigen probes is selected from the group consisting of at least a portion of a human 70 kDa heat shock protein, at least a portion of a cardiac hormone, at least a portion of a neurotransmitter, at least a portion of a pigmentation hormone, at least a portion of a vascular hormone, at least a portion of an albumin, at least a portion of a lipoprotein, at least a portion of a nerve system molecule, and at least a portion of a pancreatic molecule.

27. The antigen probe set of claim 25, wherein said plurality of antigen probes is selected from the group consisting of a bacterial heat shock protein, at least a portion of a 60 kDa human heat shock protein, at least a portion of a 70 kDa human heat shock protein, at least a portion of a metabolic enzyme, at least a portion of a lipopolysaccharide, at least a portion of a molluscan antigen, at least a portion of a wheat antigen, and at least a portion of a nerve system molecule.

28. The antigen probe set of claim 25, wherein said plurality of antigen probes is selected from the group consisting of at least a portion of a 60 kDa human heat shock protein, at least a portion of a 70 kDa human heat shock protein, at least a portion of a cytokine receptor, at least a portion of a T-cell receptor, at least a portion of a metabolic enzyme, at least a portion of a coagulation regulator, at least a portion of a nerve system molecule, and at least a portion of a pancreatic molecule.

29. The antigen probe set of claim 25, wherein said plurality of antigen probes is selected from the group consisting of at least a portion of a bacterial heat shock protein, at least a portion of a human 60 kDa heat shock protein, at least a portion of a human 70 kDa heat shock protein, and at least a portion of a glutathione S-transferase.

30. The antigen probe set of claim 25, wherein said plurality of antigen probes is selected from the group consisting of at least a portion of a muscle structure protein, at least a portion of a 70 kDa human heat shock protein, at least a portion of a homopolymeric polypeptide, at least a portion of a hypothalamic hormone, at least a portion of a neurotransmitter, at least a portion of a matrix metalloproteinase, at least a portion of a viral antigen, at least a portion of a wheat antigen, at least a portion of an albumin, and at least a portion of a lipoprotein.

31. The antigen probe set of claim 25, wherein said plurality of antigen probes is selected from the group consisting of at least a portion of a crystallin, at least a portion of a bacterial heat shock protein, at least a portion of a human 60 kDa heat shock protein, at least a portion of a human 70 kDa heat shock protein, at least a portion of a complement molecule, at least a portion of a cytokine, and at least a portion of a nucleic acid.

32. The antigen probe set of claim 25, wherein said plurality of antigen probes is selected from the group consisting of at least a portion of a human 70 kDa heat shock protein, at least a portion of a cytokine, at least a portion of a metabolic enzyme, at least a portion of a viral antigen, at least a portion of a molluscan antigen, at least a portion of an albumin, at least a portion of a lipoprotein, at least a portion of a cartilage antigen, and at least a portion of a nerve system molecule.

33. The antigen probe set of claim 25, wherein said plurality of antigen probes is selected from the group consisting of at least a portion of a bacterial heat shock protein, at least a portion of a human 60 kDa heat shock protein, at least a portion of a human 70 kDa heat shock protein, at least a portion of T-cell receptor, at least a portion of a lipopolysaccharide and at least a portion of a pancreatic molecule.