Issue defect augmentation and repair with in vitro cultured fibroblasts

• US 20050271633A1
• Filed: 07/06/2005
• Published: 12/08/2005
• Est. Priority Date: 02/20/1997
• Status: Abandoned Application

• Current Assignee: Don A. Kleinsek
• Original Assignee: Don A. Kleinsek
• Inventors: Kleinsek, Don A.
• Application Number:
  US11/175,904
• Publication Number:
  US 20050271633A1
• Time in Patent Office: Days
• US Class Current: 424/93.700
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61L 27/24 Collagen
• CPC Class Codes: A61L 27/3633 Extracellular matrix [ECM]
• CPC Class Codes: A61L 27/3641 characterised by the site of application in the body materials for artificial blood vessels A61L27/507; materials for use in artificial skin A61L27/60
• CPC Class Codes: A61L 27/3683 subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
• CPC Class Codes: A61L 27/3804 characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
• CPC Class Codes: A61L 27/3839 characterised by the site of application in the body materials for artificial blood vessels A61L27/507; materials for use in artificial skin A61L27/60
• CPC Class Codes: A61L 27/3895 using specific culture conditions, e.g. stimulating differentiation of stem cells, pulsatile flow conditions
• CPC Class Codes: A61P 11/04 for throat disorders
• CPC Class Codes: C12N 2533/54 Collagen; Gelatin
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06
• CPC Class Codes: C12N 5/0653 Adipocytes; Adipose tissue
• CPC Class Codes: C12N 5/0656 Adult fibroblasts

Abstract

Certain embodiments herein are directed to a method of treating a tissue associated with a defect in a human including wrinkles, rhytids, depressed scar, cutaneous depressions, stretch marks, hyperplasia of the lip, nasolabial fold, melolabial fold, scarring from acne vulgaris, and post-rhinoplasty irregularity. The tissue defect may be treated by introducing a plurality of in vitro cultured autologous fibroblast cells at or proximal to the defect area of the patient'"'"'s tissue. The autologous fibroblast cells may have been cultured in vitro to expand the number of fibroblast cells in at least one medium that comprises autologous serum. The autologous fibroblast cell cultures may be derived from connective tissue, dermal, fascial fibroblasts, papillary fibroblasts, and/or reticular fibroblasts.

Claims

1. A method of treating a tissue associated with a defect in a human patient using autologous materials derived from the patient, the method comprising:

introducing a plurality of autologous fibroblast cells to the tissue at, or proximal to, the defect of the patient after the plurality of autologous fibroblast cells have been cultured in vitro to expand the number of fibroblast cells in at least one medium that comprises autologous serum, wherein the tissue is associated with a defect chosen from the group consisting of wrinkles, rhytids, depressed scar, cutaneous depressions, stretch marks, hyperplasia of the lip, nasolabial fold, melolabial fold, scarring from acne vulgaris, and post-rhinoplasty irregularity.

2. The method of claim 1 wherein extracellular matrix produced in vitro by the autologous fibroblast cells is introduced into the patient in association with the plurality of autologous fibroblast cells.

3. The method of claim 1 wherein the autologous fibroblast cells are from connective tissue.

4. The method of claim 1 wherein the autologous fibroblast cells comprise lamina propria fibroblasts.

5. The method of claim 1 wherein the autologous fibroblast cells comprise fascia fibroblasts.

6. The method of claim 1 wherein the autologous fibroblast cells comprise papillary fibroblasts.

7. The method of claim 1 wherein the autologous fibroblast cells comprise reticular fibroblasts.

8. The method of claim 1 wherein extracellular matrix is introduced into the patient in association with the plurality of autologous fibroblast cells.

9. The method of claim 1 wherein collagen or modified collagen is introduced into the patient in association with the plurality of autologous fibroblast cells.

10. The method of claim 1 wherein fibronectin is introduced into the patient in association with the plurality of autologous fibroblast cells.

11. The method of claim 1 wherein hyaluronic acid is introduced into the patient in association with the plurality of autologous fibroblast cells.

12. The method of claim 1 wherein the tissue comprises a member chosen from the group consisting of lower dermis, middle dermis, upper dermis, and skin subcutaneous region.

13. The method of claim 1 wherein the issue comprises muscle tissue.