Analyzer for the simultaneous enzymatic detection of closely related analytes
First Claim
1. An analyzer for simultaneously detecting and measuring the concentration of two related analytes, said analytes being substrates for a common enzyme, comprising:
- (a) an enzymatic reaction monitoring component including a support base, a mixed electrode system consisting of a working electrode, an auxiliary electrode and a reference electrode, said mixed electrode system being supported by said support base, and an enzymatic reaction means incorporating said enzyme, said enzymatic reaction means being disposed on said mixed electrode system;
whereby, when said enzymatic reaction means is placed in contact with a liquid sample containing said two related analytes, said two related analytes chemically react with said enzyme to produce an electronic signal directly related to the concentration of each of said two related analytes in said liquid sample;
(b) a detector including a sensor, said detector being connected to said enzymatic reaction monitoring component and capable of continuously detecting and amplifying said electronic signal to produce amplified signals; and
(c) a data processor capable of converting the amplified signals into numerical data representative of the concentration of each of said two related analytes.
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Abstract
An analyzer and a method for simultaneously detecting and measuring the concentration of two related analytes, the analytes being substrates for a common enzyme. The analyzer comprises an enzymatic reaction monitoring component including a support base, a mixed electrode system consisting of a working electrode, an auxiliary electrode and a reference electrode, the mixed electrode system being supported by said support base, and an enzymatic reaction means incorporating the enzyme, the enzymatic reaction means being disposed on the mixed electrode system; whereby, when the enzymatic reaction means is placed in contact with a liquid sample containing the two related analytes, the two related analytes chemically react with the enzyme to produce an electronic signal directly related to the concentration of each of the two related analytes in said liquid sample; a detector including a sensor, the detector being connected to the enzymatic reaction monitoring component and capable of continuously detecting and amplifying said electronic signal to produce amplified signals; and a data processor capable of converting the amplified signals into numerical data representative of the concentration of each of the two related analytes.
6 Citations
45 Claims
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1. An analyzer for simultaneously detecting and measuring the concentration of two related analytes, said analytes being substrates for a common enzyme, comprising:
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(a) an enzymatic reaction monitoring component including a support base, a mixed electrode system consisting of a working electrode, an auxiliary electrode and a reference electrode, said mixed electrode system being supported by said support base, and an enzymatic reaction means incorporating said enzyme, said enzymatic reaction means being disposed on said mixed electrode system;
whereby, when said enzymatic reaction means is placed in contact with a liquid sample containing said two related analytes, said two related analytes chemically react with said enzyme to produce an electronic signal directly related to the concentration of each of said two related analytes in said liquid sample;
(b) a detector including a sensor, said detector being connected to said enzymatic reaction monitoring component and capable of continuously detecting and amplifying said electronic signal to produce amplified signals; and
(c) a data processor capable of converting the amplified signals into numerical data representative of the concentration of each of said two related analytes. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
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23. A method for simultaneously detecting and measuring the concentration of two related analytes in a sample, said related analytes being substrates for a common enzyme, wherein said enzyme reacts with said related analytes following specific different reaction kinetics, and wherein said method comprises:
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(a) reacting a plurality of reference samples having known concentrations and proportions of said related analytes, said proportions ranging from 0 to 100% of a first analyte to 100% to 0% of another related analyte, with said enzyme;
(b) establishing a kinetic profile having at least two points for each of said plurality of reference samples; and
(c) reacting a test sample comprising an unknown concentration and proportion of said related analytes with said enzyme and determining the concentration of said related compounds in said test sample using said established kinetic profiles. - View Dependent Claims (24, 25, 26)
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27. An enzymatic reaction monitoring component for simultaneously detecting and measuring the concentration of two related analytes, said analytes being substrates for a common enzyme, comprising:
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(a) a support base;
(b) a mixed electrode system consisting of a working electrode, an auxiliary electrode and a reference electrode, said mixed electrode system being supported by said support base; and
(c) an enzymatic reaction means incorporating said enzyme, said enzymatic reaction means being disposed on said mixed electrode system;
whereby, when said enzymatic reaction means is placed in contact with a liquid sample containing said two related analytes, said two related analytes chemically react with said enzyme to produce an electronic signal directly related to the concentration of each of said two related analytes in said liquid sample. - View Dependent Claims (28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45)
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Specification