Diffusion layer for an enzyme-based sensor application

• US 20060121547A1
• Filed: 12/03/2004
• Published: 06/08/2006
• Est. Priority Date: 12/03/2004
• Status: Abandoned Application

• Current Assignee: Roche Diagnostics Operations Incorporated (Roche Holding AG)
• Original Assignee: Roche Diagnostics Operations Incorporated (Roche Holding AG)
• Inventors: McIntire, Mark
• Application Number:
  US11/004,210
• Publication Number:
  US 20060121547A1
• Time in Patent Office: Days
• US Class Current: 435/14
• CPC Class Codes: C12Q 1/002 Electrode membranes
• CPC Class Codes: C12Q 1/006 for glucose

Abstract

A diffusion layer for an enzyme-based sensor application is provided, wherein the diffusion layer comprises (a) at least one polymer material, and (b) particles, typically hydrophilic particles, carrying the enzyme, the hydrophilic particles being dispersed in the at least one polymer material.

Claims

1. A diffusion layer comprising:

at least one polymer material, and

particles carrying an enzyme, wherein said particles are dispersed in said at least one polymer material.

2. The diffusion layer of claim 1, wherein said particles are hydrophilic.

3. The diffusion layer of claim 1 further comprising particles for optical isolation, wherein said particles for optical isolation are dispersed in said at least one polymer material.

4. The diffusion layer of claim 1, wherein said diffusion layer has a thickness between about 1 and about 100 μ

m.

5. The diffusion layer of claim 1, wherein said diffusion layer has a thickness between about 1 and about 50 μ

m.

6. The diffusion layer of claim 1, wherein said diffusion layer has a thickness between about 1 and about 20 μ

m.

7. An enzyme-based sensor comprising a diffusion layer according to claim 1.

8. The enzyme-based sensor of claim 7 comprising at least one dye layer.

9. The enzyme-based sensor of claim 7, wherein said diffusion layer according to claim 1 is the cover layer.

10. The enzyme-based sensor of claim 7, wherein said sensor is an electrochemical sensor or an optical sensor.

11. Use of an enzyme-based sensor according to claim 7 for the detection and/or qualitative and/or quantitative determination of an enzyme substrate and/or co-substrate.

12. Use according to claim 11, wherein said enzyme substrate is glucose.

13. Use according to claim 12, wherein said determination is performed in blood.

14. Use according to claim 11, wherein multiple measurements are performed.

15. A method of preparing a diffusion layer for an enzyme-based sensor comprising:

(i) forming a dispersion comprising at least one polymer material and enzyme-carrying partricles;

(ii) applying said dispersion directly on an underlying layer to form an enzyme-carrying diffusion layer; and

(iii) drying the dispersion.

16. The method of claim 15, wherein the drying comprises removing a solvent from the dispersion.