Method for the production of porous carbon-based molded bodies, and use thereof as cell culture carrier systems and culture systems

• US 20060159718A1
• Filed: 01/30/2006
• Published: 07/20/2006
• Est. Priority Date: 07/31/2003
• Status: Abandoned Application

• Current Assignee: Cinvention AG
• Original Assignee: Cinvention AG
• Inventors: Rathenow, Jorg, Kunstmann, Jurgen, Asgari, Soheil
• Application Number:
  US11/343,480
• Publication Number:
  US 20060159718A1
• Time in Patent Office: Days
• US Class Current: 424/422
• CPC Class Codes: A61L 27/08 Carbon ; Graphite
• CPC Class Codes: A61L 27/38 containing added animal cells organs or tissue containing native cells A61L27/36
• CPC Class Codes: A61L 27/56 Porous materials, e.g. foams or sponges
• CPC Class Codes: B01D 2325/02 Details relating to pores or porosity of the membranes
• CPC Class Codes: B01D 2325/0283 Pore size
• CPC Class Codes: B01D 67/0072 by deposition from the gaseous phase, e.g. sputtering, CVD, PVD
• CPC Class Codes: B01D 67/0088 Physical treatment with compounds, e.g. swelling, coating or impregnation
• CPC Class Codes: B01D 69/04 Tubular membranes
• CPC Class Codes: B01D 71/021 Carbon
• CPC Class Codes: B01J 21/18 Carbon
• CPC Class Codes: B01J 37/0018 Addition of a binding agent or of material, later completely removed among others as result of heat treatment, leaching or washing,(e.g. forming of pores; protective layer, desintegrating by heat)
• CPC Class Codes: B01J 37/084 Decomposition of carbon-containing compounds into carbon
• CPC Class Codes: C04B 2103/0067 the ingredients being formed in situ by chemical reactions or conversion of one or more of the compounds of the composition
• CPC Class Codes: C04B 2103/0099 Aspecific ingredients, i.e. high number of alternative specific compounds mentioned for the same function or property
• CPC Class Codes: C04B 2111/00612 as one or more layers of a layered structure
• CPC Class Codes: C04B 2111/0081 as catalysts or catalyst carriers
• CPC Class Codes: C04B 2111/00836 for medical or dental applications
• CPC Class Codes: C04B 2111/56 Compositions suited for fabrication of pipes, e.g. by centrifugal casting, or for coating concrete pipes
• CPC Class Codes: C04B 2235/3418 Silicon oxide, silicic acids, or oxide forming salts thereof, e.g. silica sol, fused silica, silica fume, cristobalite, quartz or flint glass constituents C04B2235/36
• CPC Class Codes: C04B 2235/663 Oxidative annealing
• CPC Class Codes: C04B 2235/77 : Density
• CPC Class Codes: C04B 2235/94 : Products characterised by their shape
• CPC Class Codes: C04B 35/52 : based on carbon, e.g. graphite
• CPC Class Codes: C04B 35/524 : obtained from polymer precursors, e.g. glass-like carbon material
• CPC Class Codes: C04B 35/64 : Burning or sintering processes C04B33/32 takes precedence ; powder metallurgy B22F
• CPC Class Codes: C04B 35/80 : Fibres, filaments, whiskers, platelets, or the like
• CPC Class Codes: C04B 35/82 : Asbestos; Glass; Fused silica
• CPC Class Codes: C04B 35/83 : Carbon fibres in a carbon matrix
• CPC Class Codes: C04B 38/0032 : one of the precursor materials being a monolithic element having approximately the same dimensions as the final article, e.g. a paper sheet which after carbonisation will react with silicon to form a porous silicon carbide porous body
• CPC Class Codes: C04B 38/04 : by dissolving-out added substances
• CPC Class Codes: C04B 41/45 : Coating or impregnating paints C09D, e.g. injection in masonry, partial coating of green or fired ceramics, organic coating compositions for adhering together two concrete elements ion-implantation C04B41/0027
• CPC Class Codes: C07K 16/00 : Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies antibodies with enzymatic activity, e.g. abzymes C12N9/0002
• CPC Class Codes: C07K 16/1203 : from Gram-negative bacteria
• CPC Class Codes: C12M 25/14 : Scaffolds; Matrices in general C12N5/0068
• CPC Class Codes: C12N 2533/10 : Mineral substrates
• CPC Class Codes: C12N 5/0068 : General culture methods using substrates for specific animal cell type C12N5/06

Abstract

The present invention relates to methods for producing carbon-based molded bodies. In particular, the present invention relates to methods for producing porous carbon-based molded bodies by carbonizing organic polymer materials mixed with non-polymeric fillers and subsequently dissolving the fillers out from the carbonized molded bodies. The present invention further relates to methods for producing porous carbon-based molded bodies by carbonizing organic polymer materials mixed with non-polymeric fillers which are substantially completely decomposed during the carbonization. The present invention also relates to a method for producing porous carbon-based molded bodies by carbonizing organic polymer materials, the carbon-based molded bodies being partially oxidized following carbonization so as to produce pores. In addition, the present invention relates to porous molded bodies produced according to one of said methods and the use thereof, especially as cell culture carriers and/or culture systems.

Claims

1. A method for producing porous molded bodies comprising:

(a) forming at least one first molded part from a composition comprising at least one organic polymer material;

(b) carbonizing the at least one first molded part in a non-oxidizing atmosphere to form at least one second molded part; and

(c) producing at least one molded body with pores from the at least one second molded part.

2. The method of claim 1, wherein the composition further comprises at least one polymeric filler, and wherein steps (b) and (c) are performed simultaneously, and wherein the at least one polymeric filler is at least substantially decomposed.

3. The method of claim 2, wherein the at least one polymeric filler comprises at least one of saturated aliphatic hydrocarbon homopolymers, branched aliphatic hydrocarbon homopolymers unbranched aliphatic hydrocarbon homopolymers, saturated aliphatic hydrocarbon copolymers, branched aliphatic hydrocarbon copolymers unbranched aliphatic hydrocarbon copolymers, polyolefins, polyethylene, polypropylene, polybutene, polyisobutene, or polypentene.

4. The method of claim 1, wherein the at least one organic polymer material comprises at least one of unsaturated branched aliphatic hydrocarbons, branched cross-linked aromatic hydrocarbons, unbranched cross-linked aromatic hydrocarbons, branched non-cross-linked aromatic hydrocarbons, unbranched non-cross-linked aromatic hydrocarbons, branched cross-linked partially aromatic hydrocarbons, unbranched cross-linked partially aromatic hydrocarbons, branched non-cross-linked partially aromatic hydrocarbons, unbranched non-cross-linked partially aromatic hydrocarbons, or substituted derivatives of any of the preceding.

5. The method of claim 1, further comprising treating the at least one molded body with at least one of an oxidizing agent or a reducing agent.

6. The method of claim 1, wherein step (c) comprises partially oxidizing the at least one second molded part to produce pores therein.

7. The method of claim 6, wherein step (c) further comprises heat treating the at least one second molded part in an oxidizing gas atmosphere.

8. The method of claim 7, wherein the oxidizing gas atmosphere comprises at least one of air, oxygen, carbon monoxide, carbon dioxide or a nitrogen oxide, and wherein step (c) is performed at temperatures in the range of about 50 to 800°

C.

9. The method of claim 6, wherein step (c) comprises exposing the at least one second molded part to oxidizing acids.

10. The method of claim 1, wherein the at least one organic polymer material comprises at least one of polybutadiene, a polyvinyl, polyvinylchloride, polyvinyl alcohol, poly(meth)acrylic acid, polyacryl cyanoacrylate, polyacrylnitrile, polyamide, polyester, polyurethane, polystyrene, polytetrafluoroethylene, collagen, albumin, gelatin, hyaluronic acid, starch, cellulose, methylcellulose, hydroxypropylmethyl cellulose, carboxymethyl cellulose phthalate, casein, dextran, polysaccharide fibrinogen, poly(D,L-lactide), poly(D,L-lactide-co-glycolide), polyglycolide, polyhydroxybutylate, polyalkylcarbonate, polyorthoester, polyester, polyhydroxyvaleric acid, polydioxanone, polyethylene terephthalate, polymalic acid, polytartaric acid, polyanhydride, polyphosphazene, polyamino acids;

polyethylenevinyl acetate, silicone;

poly(ester urethane), poly(ether urethane), poly(ester urea), a polyether, polyethylene oxide, polypropylene oxide, pluronics, polytetramethylene glycol, polyvinyl pyrrolidone, poly(vinyl acetate phthalate), alkyd resin, chlororubber, epoxy resin, acrylate resin, phenol resin, amine resin, melamine resin, an alkylphenol resin, an epoxided aromatic resin, tar, a tar-like material, tar pitch, a liquid-crystal tar pitch, bitumen, starch, cellulose, shellac, polyacrylnitrile fibers, cellulose fibers, novolak fibers, organic materials of renewable raw materials, or copolymers of any of the preceding.

11. The method of claim 1, wherein the composition further comprises at least one of a filler, a softener, a lubricant, a flame retardant, glass, glass fibers, carbon fibers, cotton, fabric, metal powder, metal compounds, metal oxides, silicon, silicon oxide, zeolites, TiO₂, aluminium oxide, aluminosilicate, zirconium oxide, talc, graphite, soot, clay materials, or phyllosilicates.

12. The method of claim 1, wherein step (a) comprises at least one of casting, extruding, pressing, embossing, or injection molding the composition.

13. The method of claim 1, wherein step (b) is carried out under a protective gas atmosphere.

14. The method of claim 13, wherein the protective gas atmosphere comprises at least one of nitrogen or argon.

15. The method of claim 13, wherein the protective gas atmosphere comprises a reactive gas.

16. The method of claim 15, wherein the reactive gas is hydrogen.

17. The method of claim 1, wherein step (b) is performed at temperatures in the range of about 200 to 4000°

C.

18. A porous molded body produced by:

(a) forming at least one first molded part from a composition comprising at least one organic polymer material;

(b) carbonizing the at least one first molded part in a non-oxidizing atmosphere to form at least one second molded part; and

(c) producing the molded body with pores from the at least one second molded part.

19. The molded body of claim 18, wherein the molded body is in the shape of at least one of a tube, a round rod, a plate, a block, a rectangular parallelepiped, a cube, an injection mold, a honeycomb structure, an imprinted structure, a folded structure, a wound structure, a rolled two-dimensional structure, a rolled three-dimensional structure, a channeled structure, a solid sphere, a hollow sphere, a flange, a seal, or a housing.

20. The molded body of claim 18, wherein the molded body is configured to be used as at least one of a carrier or a culture system for the cultivation of primary cell cultures.

21. The molded body of claim 20, wherein the cell cultures comprise at least one of eukaryotic tissue, bone, cartilage, liver cells, kidney cells, pancreas cells, nerves, xenogenic cells, allogenic cells, syngenic cells, autologous cells, or genetically modified cell lines.

22. The molded body of claim 21, wherein the molded body is configured to be used as a guide structure for growth of tissue.

23. The molded body of claim 22, wherein the tissue comprises at least part of a bodily organ.

24. The molded body of claim 19, wherein the molded body is configured to be used as an ex vivo reactor system.

25. The molded body of claim 20, wherein the molded body is configured to be used in vivo as an implant.

26. The molded body of claim 20, wherein the molded body is modified with at least one of proteoglycans, collagens, tissue-type salts, growth factors, biologically degradable polymers, or resorbable polymers.