Method for the characterization of the three-dimensional structure of proteins employing mass spectrometric analysis and computational feedback modeling
First Claim
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1. A method for obtaining spectral data, comprising the steps of:
- (a) mixing a small molecule with a large molecule so that the small molecule binds non-covalently to the large molecule to form a large molecule/small molecule complex; and
(b) performing electrospray ionization mass spectrometry to obtain spectral data relating to the large molecule-small molecule complex.
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Abstract
A method for characterizing the three-dimensional surface structure of molecules, particularly proteins and protein complexes, employing mass spectrometric analysis, an electrospray ionization (ES) source, a novel data interpretation process that utilizes comparisons of particular binding constants (KB) and heats of formation (ΔHf), and computational feedback modeling.
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8 Claims
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1. A method for obtaining spectral data, comprising the steps of:
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(a) mixing a small molecule with a large molecule so that the small molecule binds non-covalently to the large molecule to form a large molecule/small molecule complex; and
(b) performing electrospray ionization mass spectrometry to obtain spectral data relating to the large molecule-small molecule complex. - View Dependent Claims (2, 3, 4, 5)
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6. A method for obtaining spectral data, comprising the steps of:
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(a) mixing a crown ether with a large molecule so that the crown ether binds non-covalently to the large molecule to form a large molecule/crown ether complex; and
(b) performing electrospray ionization mass spectrometry to obtain spectral data relating to the large molecule/crown either complex. - View Dependent Claims (7, 8)
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Specification