Devices and Methods for Controlling Bubble Formation in Microfluidic Devices

• US 20070280856A1
• Filed: 06/02/2006
• Published: 12/06/2007
• Est. Priority Date: 06/02/2006
• Status: Abandoned Application

• Current Assignee: Applied Biosystems LLC (Thermo Fisher Scientific Incorporated)
• Original Assignee: Applera Corporation (Thermo Fisher Scientific Incorporated)
• Inventors: Beard, Nigel P., Ulmanella, Umberto, Liu, David, Yue, Min, Nordman, Eric S., Yang, Joon Mo, Schembri, Carol, Song, Maengseok, Lee, Julie C.
• Application Number:
  US11/422,057
• Publication Number:
  US 20070280856A1
• Time in Patent Office: Days
• US Class Current: 422/100
• CPC Class Codes: B01L 2200/0642 Filling fluids into wells by specific techniques
• CPC Class Codes: B01L 2200/0673 Handling of plugs of fluid surrounded by immiscible fluid
• CPC Class Codes: B01L 2200/0684 Venting, avoiding backpressure, avoid gas bubbles
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 2400/0409 centrifugal forces
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 2400/086 using baffles or other fixed flow obstructions
• CPC Class Codes: B01L 3/502723 characterised by venting arrangements
• CPC Class Codes: B01L 3/502746 characterised by the means for controlling flow resistance, e.g. flow controllers, baffles B01L3/502738 takes precedence
• CPC Class Codes: B01L 3/502784 specially adapted for droplet or plug flow, e.g. digital microfluidics automatic analysis using a stream of discrete samples in a tube system G01N35/08

Abstract

A microfluidic device may include a sample distribution network including a plurality of sample chambers configured to be loaded with biological sample for biological testing of the biological sample while in the sample chambers, the biological sample having a meniscus that moves within the sample chambers during loading. The sample distribution network may further include a plurality of inlet channels, each inlet channel being in flow communication with and configured to flow biological sample to a respective sample chamber, and a plurality of outlet channels, each outlet channel being in flow communication and configured to flow biological sample from a respective sample chamber. At least some of the sample chambers may include a physical modification configured to control the movement of the meniscus so as to control bubble formation within the at least some sample chambers. At least some of the sample chambers may include a dried reagent positioned within the at least some sample chambers proximate the inlet channels in flow communication with the at least some sample chambers.

Claims

1. A microfluidic device, comprising:

a sample distribution network comprising;

a plurality of sample chambers configured to be loaded with biological sample for biological testing of the biological sample while in the sample chambers, the biological sample having a meniscus that moves within the sample chambers during loading,

a plurality of inlet channels, each inlet channel being in flow communication with and configured to flow biological sample to a respective sample chamber, and

a plurality of outlet channels, each outlet channel being in flow communication with and configured to flow biological sample from a respective sample chamber,

wherein at least some of the sample chambers comprise at least one physical modification configured to control the movement of the meniscus so as to control bubble formation within the at least some sample chambers.

2. The microfluidic device of claim 1, wherein the at least one physical modification is configured to control the movement of the meniscus such that differing portions of the meniscus move at substantially the same rate.

3. The microfluidic device of claim 1, wherein the at least one physical modification is configured to control the movement of the meniscus by altering a rate of movement of a portion of the meniscus relative to another portion of the meniscus.

4. The microfluidic device of claim 1, wherein the at least one physical modification is configured to control the movement of the meniscus such that substantially all portions of the meniscus reach the respective outlet channels in flow communication with each of the at least some sample chambers at substantially the same time.

5. The microfluidic device of claim 1, wherein the at least one physical modification comprises at least one of a groove, a feature in relief, and a projecting member.

6. The microfluidic device of claim 5, wherein the at least one physical modification comprises at least one projecting member chosen from teeth and pillars.

7. The microfluidic device of claim 1, wherein the at least one physical modification comprises an interior surface portion of the at least some chambers that joins an interior surface portion of the inlet channels and outlet channels in flow communication with each of the at least some sample chambers at a nonperpendicular angle.

8. The microfluidic device of claim 1, wherein the at least one physical modification comprises a variable depth of the at least some chambers.

9. The microfluidic device of claim 1, wherein the at least one physical modification comprises at least one expanded opening to at least one of the inlet channels and the outlet channels in flow communication with the at least some chambers.

10. The microfluidic device of claim 1, wherein the at least one physical modification comprises an elongated shape of the at least some chambers.

11. The microfluidic device of claim 1, wherein the at least one physical modification is configured to passively control the movement of the meniscus.

12. The microfluidic device of claim 1, wherein the sample-distribution network further comprises at least one main channel and wherein the plurality of sample chambers are in flow communication with the at least one main channel via the plurality of inlet channels.

13. The microfluidic device of claim 1, wherein each of the plurality of sample chambers comprises the at least one physical modification.

14. The microfluidic device of claim 1, wherein the sample distribution network is supplied with biological sample via pressure filling.

15. The microfluidic device of claim 1, wherein the at least one physical modification comprises a dried reagent positioned within the at least some sample chambers.

16. A method of filling a microfluidic device, the method comprising:

supplying the microfluidic device with a biological sample, the microfluidic device comprising

a plurality of sample chambers,

a plurality of inlet channels, each inlet channel being in flow communication with and configured to flow biological sample to a respective sample chamber, and

a plurality of outlet channels, each outlet channel being in flow communication with and configured to flow biological sample from a respective sample chamber;

loading the sample chambers with the biological sample, the biological sample having a meniscus that moves within the sample chambers as the biological sample loads the sample chambers; and

during loading, controlling the movement of the meniscus via at least one physical modification of at least some of the sample chambers so as to control bubble formation within the at least some sample chambers.

17. The method of claim 16, wherein controlling the movement of the meniscus comprises controlling the movement of the meniscus such that differing portions of the meniscus move at substantially the same rate.

18. The method of claim 16, wherein controlling the movement of the meniscus comprises altering a rate of movement of a portion of the meniscus relative to another portion of the meniscus.

19. The method of claim 16, wherein the controlling the movement of the meniscus comprises passively controlling the movement of the meniscus.

20. The method of claim 16, wherein controlling the movement of the meniscus via the at least one physical modification comprises controlling the movement of the meniscus via at least one physical modification chosen from at least one of a groove, a feature in relief, and a projecting member.

21. The method of claim 16, wherein controlling the movement of the meniscus via the at least one physical modification comprises controlling the movement of the meniscus via an interior surface portion of the at least some chambers that joins an interior surface portion of the inlet channel and outlet channel in flow communication with the at least some chambers at a nonperpendicular angle.

22. The method of claim 16, wherein controlling the movement of the meniscus via the at least one physical modification comprises controlling the movement of the meniscus via a variable depth of the at least some chambers.

23. The method of claim 16, wherein controlling the movement of the meniscus via the at least one physical modification comprises controlling the movement of the meniscus via at least one expanded opening to at least one of the inlet channel and the outlet channel in flow communication with the at least some chambers.

24. The method of claim 16, wherein controlling the movement of the meniscus via the at least one physical modification comprises controlling the movement of the meniscus via an expansion ratio associated with at least one of the inlet channel and the outlet channel in flow communication with the at least some sample chambers.

25. The method of claim 16, wherein controlling the movement of the meniscus via the at least one physical modification comprises controlling the movement of the meniscus via an elongated shape of the at least some chambers.

26. The method of claim 16, wherein controlling the movement of the meniscus comprises controlling the movement of the meniscus via at least one physical modification of each of the plurality of sample chambers.

27. The method of claim 16, wherein supplying the microfluidic device with the biological sample comprises supplying the microfluidic device with the biological sample via pressure filling.

28. The method of claim 16, wherein controlling the movement of the meniscus via the at least one physical modification comprises controlling the movement of the meniscus via a dried reagent positioned within the at least some sample chambers.