Hydrogel for an intravenous amperometric biosensor
First Claim
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1. A hydrogel layer for an amperometric biosensor, comprising:
- chitosan;
at least one reactive agent; and
at least one cross-linking agent cross-linked to the chitosan forming a matrix immobilizing the at least one reactive agent.
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Abstract
A hydrogel for an intravenous amperometric biosensor is formed on an electrode from a matrix of chitosan and a cross-linking agent, such as genipin to immobilize a reactive agent such as glucose oxidase. The matrix may also function as a rate-limiting layer to selectively control diffusion to the reactive agent. A method for forming the hydrogel on an electrode for immobilizing a reactive agent may include dissolving chitosan in an acidic solution, adding the reactive agent, applying solution to the electrode surface, curing the solution to a solid film, and immersing the same in a genipin buffer.
128 Citations
20 Claims
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1. A hydrogel layer for an amperometric biosensor, comprising:
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chitosan;
at least one reactive agent; and
at least one cross-linking agent cross-linked to the chitosan forming a matrix immobilizing the at least one reactive agent. - View Dependent Claims (2, 3, 4)
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5. An intravenous amperometric biosensor, comprising:
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a flexible substrate;
an electrode bonded to the substrate; and
a hydrogel layer bonded to the electrode, the hydrogel layer including chitosan, at least one reactive agent, and at least one cross-linking agent cross-linked to the chitosan forming a matrix immobilizing the at least one reactive agent. - View Dependent Claims (6, 7, 8, 9, 10)
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11. A method for forming a hydrogel for immobilizing a reactive agent on an electrode, comprising:
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dissolving chitosan in an acidic solution;
adding a reactive agent to the chitosan solution;
applying the chitosan solution to a surface of the electrode;
curing the applied chitosan solution until it forms a solid film; and
immersing the solid film in a genipin solution to cross-link the chitosan and the genipin, thereby immobilizing the reactive agent. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19, 20)
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Specification