DROPLET DETECTION SYSTEM
First Claim
Patent Images
1. The droplet detection device for monitoring a dispensing operation in a high throughput system, said device comprising:
- a plate with a predetermined number of wells and well walls;
an image capturing device to extract image features from said wells and/or well walls; and
a control processor for analyzing said extracted image features to determine the presence of dispensing fluid on said wells and said well walls.
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Abstract
A system for a droplet detection device to monitor the dispensing operation in an ultra high throughput (uHTS) system. The system includes a microtitre plate with a defined number of wells and well walls, a dispenser for dispensing fluids into the wells, an illuminating sensor to extract image features from the wells and well walls, and a controller for analyzing the extracted image features to determine the presence or absence of the dispensing fluid on the wells and well walls.
8 Citations
18 Claims
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1. The droplet detection device for monitoring a dispensing operation in a high throughput system, said device comprising:
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a plate with a predetermined number of wells and well walls; an image capturing device to extract image features from said wells and/or well walls; and a control processor for analyzing said extracted image features to determine the presence of dispensing fluid on said wells and said well walls. - View Dependent Claims (2, 3, 4, 5, 6)
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7. The droplet detection device for monitoring dispensing operations in a high throughput system, said device comprising:
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a plate with a predetermined number of wells and well walls; one or more cameras each with built-in electronics to extract image features from said wells and well walls; a pair of illumination devices adjacent to said cameras, respectively, to distribute reflected light onto said plate; and a control processor for analyzing said extracted image features to determine the presence of said dispensing fluid on said wells and said well walls. - View Dependent Claims (8, 9, 10, 11, 12)
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13. A method for detecting droplets dispensed in a plate with a plurality of wells in a high throughput system, said method comprising:
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dispensing fluids into said wells of said plate; illuminating said plate with reflected light; extracting image features from said wells and well walls; and processing said extracted image features to determine the presence of said dispensing fluid on said wells and said well walls. - View Dependent Claims (14, 15, 16, 17)
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18. A computer readable recording medium having recorded thereon a program for causing a computer to perform the steps of:
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interfacing with an operator, camera, external device, or motion controller; determining one or more positions of a plate and acquiring one or more images of the plate; aligning one or more image windows onto said one or more images; analyzing said one or more images to detect droplets and full or empty wells; controlling information transfer between a frame grabber and said camera; and notifying the operator based on notification criteria.
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Specification