INDIRECTLY LABELLED ASSAY CONJUGATES AND METHODS OF PREPARING AND USING SAME
First Claim
1. A process for preparing a protein conjugate, said process comprising the steps of:
- (a) subjecting a protein to denaturing conditions to provide a treated protein; and
(b) conjugating said treated protein to a label moiety to provide said indirectly labelled protein conjugate, said label moiety comprising a carrier molecule coupled to one or more detectable labels.
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Accused Products
Abstract
Indirectly labelled assay conjugates prepared by a method that includes the step of submitting the binding member comprised by the conjugate to denaturing conditions prior to labelling the binding member. The indirectly labelled assay conjugates demonstrate an increased sensitivity when employed in diagnostic assays compared to assay conjugates prepared by methods that do not include a step of submitting the binding member to denaturing conditions prior to labelling. Processes for the preparation of the indirectly labelled assay conjugates, methods of detecting an analyte comprising the use of the indirectly labelled assay conjugate and kits comprising the indirectly labelled conjugates are also provided.
24 Citations
38 Claims
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1. A process for preparing a protein conjugate, said process comprising the steps of:
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(a) subjecting a protein to denaturing conditions to provide a treated protein; and (b) conjugating said treated protein to a label moiety to provide said indirectly labelled protein conjugate, said label moiety comprising a carrier molecule coupled to one or more detectable labels. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 34)
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- 33. A protein conjugate comprising a hepatitis C virus NS3 antigen conjugated to a label moiety, said label moiety comprising a carrier moiety coupled to at least one detectable label.
Specification