COMPONENTS OF CELL CULTURE MEDIA PRODUCED FROM PLANT CELLS
First Claim
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1. A cell culture media, comprising at least one plant-produced heterologous protein as a cell culture media component.
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Abstract
The present invention comprises the production of components of cell culture media produced from plant cells and cell culture media containing the same. Heterologous DNA comprising genes encoding the desired component are introduced into plant cells, especially rice, which then produce the desired component. The component can be isolated from the plant cell and combined with other components to form the required cell culture medium.
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19 Claims
- 1. A cell culture media, comprising at least one plant-produced heterologous protein as a cell culture media component.
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14. A method for producing an enhanced cell culture media, comprising producing at least one heterologous protein in a plant cell and combining the at least one plant-produced heterologous protein with a cell culture media.
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15. A cell culture media produced by a process comprising producing at least one heterologous protein in a plant cell and combining the at least one plant-produced heterologous protein with a cell culture media.
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16. In a cell culture media having one or more proteinaceous cell culture media components, the improvement comprising at least one plant-produced heterologous protein as a cell culture media component, wherein the heterologous protein is produced by the following process:
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a) transforming a plant cell with a chimeric gene comprising; (i) a promoter from the gene of a seed storage protein; (ii) a first DNA sequence, operably linked to the promoter, encoding a signal sequence of a seed storage protein, and (iii) a second DNA sequence, operably linked to the promoter, encoding the heterologous protein, wherein the first and second DNA sequences are linked in translation frame and together encode a fusion protein comprising the storage protein and the heterologous protein; and b) growing a plant from the transformed plant cell for a time sufficient to produce seeds containing the heterologous protein.
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- 17. The method of claim 17, further comprising purifying the heterologous protein from the harvested seeds.
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19. A method for producing a cell culture media having at least one plant-produced heterologous protein as a cell culture media component, the method comprising:
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a) transforming a plant cell with a chimeric gene comprising; (i) a promoter from the gene of a seed storage protein; (ii) a first DNA sequence, operably linked to the promoter, encoding a signal sequence of a seed storage protein, and (iii) a second DNA sequence, operably linked to the promoter, encoding the heterologous protein, wherein the first and second DNA sequences are linked in translation frame and together encode a fusion protein comprising the storage protein and the heterologous protein; b) growing a plant from the transformed plant cell for a time sufficient to produce seeds containing the heterologous protein; c) harvesting the seeds from the plant; and d) combining the heterologous protein with a cell culture media.
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Specification