Ketoreductases and Uses Thereof
1 Assignment
0 Petitions
Accused Products
Abstract
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.
30 Citations
54 Claims
-
1-36. -36. (canceled)
-
37. An isolated or engineered polynucleotide encoding a recombinant polypeptide capable of converting acetophenone to (R)-1-phenylethanol which comprises an amino acid sequence with at least 90% sequence identity to SEQ ID NO:
- 2 or SEQ ID NO;
4 and having (a) an aromatic amino acid or G at the amino acid residue corresponding to residue 94 of SEQ ID NO;
2 or SEQ ID NO;
4, or (b) an amino acid other than S and N at the amino acid residue corresponding to residue 96 of SEQ ID NO;
2 or SEQ ID NO;
4. - View Dependent Claims (38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54)
- 2 or SEQ ID NO;
Specification