PYRIDINIUM BORONIC ACID QUENCHERS FOR USE IN ANALYTE SENSORS

• US 20110171742A1
• Filed: 03/22/2011
• Published: 07/14/2011
• Est. Priority Date: 05/01/2007
• Status: Active Grant

• Current Assignee: Medtronic Minimed Incorporated (Medtronic Plc)
• Original Assignee: GluMetrics, Inc.
• Inventors: GAMSEY, SOYA, WESSLING, RITCHIE A.
• Granted Patent: US 8,178,676 B2
• Time in Patent Office: Days
• US Class Current: 436/95
• CPC Class Codes: C07F 5/025 Boronic and borinic acid compounds
• CPC Class Codes: G01N 2400/00 Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
• CPC Class Codes: G01N 33/523 the element being adapted for a specific analyte
• CPC Class Codes: Y10T 436/104998 Glucose, ketone, nitrate standard or control
• CPC Class Codes: Y10T 436/144444 Glucose
• CPC Class Codes: Y10T 436/145555 Hetero-N

Abstract

Novel pyridinium salts functionalized with boronic acid and methods of making them are disclosed. When combined with a fluorescent dye, the compounds are useful in the detection of polyhydroxyl-substituted organic molecules.

Claims

1. The compound:

2. A method of making the compound of claim 1, comprising the steps of:

6. A glucose sensor comprising the compound of claim 1 and a fluorescent dye.

7. The glucose sensor of claim 6, wherein said compound is in the form of a polymer.

8. The glucose sensor of claim 6, further comprising a glucose permeable immobilizing means.

9. The glucose sensor of claim 8, wherein said glucose permeable immobilizing means is a polymer matrix or a semipermeable membrane.

3. The compound:

4. A method of making the compound of claim 3, comprising the steps of:

10. A glucose sensor comprising the compound of claim 3 and a fluorescent dye.

11. The glucose sensor of claim 10, wherein said compound is in the form of a polymer.

12. The glucose sensor of claim 10, further comprising a glucose permeable immobilizing means.

13. The glucose sensor of claim 12, wherein said glucose permeable immobilizing means is a polymer matrix or a semipermeable membrane.

5. An analyte sensor comprising:

a fluorophore configured to absorb light at a first wavelength and emit light at a second wavelength; and

a quencher configured to modify the light emitted by the fluorophore by an amount related to the analyte concentration, wherein said quencher comprises three or more pyridinium groups.