Pyridinium boronic acid quenchers for use in analyte sensors

• US 8,178,676 B2
• Filed: 03/22/2011
• Issued: 05/15/2012
• Est. Priority Date: 05/01/2007
• Status: Active Grant

• Current Assignee: Medtronic Minimed Incorporated (Medtronic Plc)
• Original Assignee: GluMetrics, Inc.
• Inventors: Gamsey, Soya, Wessling, Ritchie A.
• Primary Examiner(s): Aulakh, Charanjit
• Application Number:
  US13/069,150
• Publication Number:
  US 20110171742A1
• Time in Patent Office: 420 Days
• Field of Search: 546/13, 436/14, 424/9.1
• US Class Current: 546/13
• CPC Class Codes: C07F 5/025 Boronic and borinic acid compounds
• CPC Class Codes: G01N 2400/00 Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
• CPC Class Codes: G01N 33/523 the element being adapted for a specific analyte
• CPC Class Codes: Y10T 436/104998 Glucose, ketone, nitrate standard or control
• CPC Class Codes: Y10T 436/144444 Glucose
• CPC Class Codes: Y10T 436/145555 Hetero-N

Abstract

Novel pyridinium salts functionalized with boronic acid and methods of making them are disclosed. When combined with a fluorescent dye, the compounds are useful in the detection of polyhydroxyl-substituted organic molecules.

Claims

1. The compound:

2. A method of making the compound of claim 1, comprising the steps of:

5. A glucose sensor comprising the compound of claim 1 and a fluorescent dye.

6. The glucose sensor of claim 5, wherein said compound is in the form of a polymer.

7. The glucose sensor of claim 5, further comprising a glucose permeable immobilizing means.

8. The glucose sensor of claim 7, wherein said glucose permeable immobilizing means is a polymer matrix or a semipermeable membrane.

3. The compound:

4. A method of making the compound of claim 3, comprising the steps of:

9. A glucose sensor comprising the compound of claim 3 and a fluorescent dye.

10. The glucose sensor of claim 9, wherein said compound is in the form of a polymer.

11. The glucose sensor of claim 9, further comprising a glucose permeable immobilizing means.

12. The glucose sensor of claim 11, wherein said glucose permeable immobilizing means is a polymer matrix or a semipermeable membrane.