GENETIC ANALYSIS LOC WITH LOW OLIGONUCLEOTIDE PROBE MASS AND LOW REAGENT VOLUME
First Claim
1. A lab-on-a-chip (LOC) device for genetic analysis comprising:
- a supporting substrate;
a microsystems technology (MST) layer supported on the substrate, the MST layer having an inlet for receiving a biological sample containing target nucleic acid sequences;
a hybridization section comprising an array of chambers with probes for hybridization with the target nucleic acid sequences to form probe-target hybrids; and
,a reagent reservoir containing a reagent necessary for processing the biological sample prior to hybridization;
wherein,the hybridization section contains less than 270 picograms of each probe per chamber and the reagent reservoir containing less than 1000,000,000 cubic microns of the reagent.
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Accused Products
Abstract
A lab-on-a-chip (LOC) device for genetic analysis having a supporting substrate, a microsystems technology (MST) layer supported on the substrate, the MST layer having an inlet for receiving a biological sample containing target nucleic acid sequences, a hybridization section comprising an array of chambers with probes for hybridization with the target nucleic acid sequences to form probe-target hybrids, and, a reagent reservoir containing a reagent necessary for processing the biological sample prior to hybridization, wherein, the hybridization section contains less than 270 picograms of each probe per chamber and the reagent reservoir containing less than 1000,000,000 cubic microns of the reagent.
4 Citations
20 Claims
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1. A lab-on-a-chip (LOC) device for genetic analysis comprising:
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a supporting substrate; a microsystems technology (MST) layer supported on the substrate, the MST layer having an inlet for receiving a biological sample containing target nucleic acid sequences; a hybridization section comprising an array of chambers with probes for hybridization with the target nucleic acid sequences to form probe-target hybrids; and
,a reagent reservoir containing a reagent necessary for processing the biological sample prior to hybridization;
wherein,the hybridization section contains less than 270 picograms of each probe per chamber and the reagent reservoir containing less than 1000,000,000 cubic microns of the reagent. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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Specification