Process for the Culturing of Cells
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Abstract
The invention relates to a process for the culturing of cells, preferably El-immortalized HER cells, more preferably PER.C6 cells in a reactor in suspension in a cell culture medium, wherein the cells produce a biological substance, preferably an antibody, wherein at least one cell culture medium component is fed to the cell culture and wherein the cell culture comprising the cells, the biological substance and cell culture medium is circulated over a separation system and wherein the separation system separates the biological substance from substances having a lower molecular weight than the biological substance and wherein the biological substance is retained in or fed back into the reactor. Preferably part of the substances of lower molecular weight is continuously removed from the cell culture.
3 Citations
23 Claims
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1-14. -14. (canceled)
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15. A cell culture comprising
(a) cell culture media; -
(b) animal cells having a viable cell density of at least 50×
106suspended cells/mL of cell culture media; and(c) a biological substance having a concentration of at least 5 g/L of cell culture media; wherein the cell culture is within or directly obtained from a reactor. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22, 23)
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Specification