Protein purification method

  • US 7,927,815 B2
  • Filed: 01/23/2008
  • Issued: 04/19/2011
  • Est. Priority Date: 03/09/2001
  • Status: Active Grant
First Claim
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1. A method for removing contaminant DNA in a sample containing a physiologically active protein, which comprises the following steps:

  • 1) converting the sample containing a physiologically active protein into an acidic aqueous solution of low conductivity of 300 mS/m or less and having a molarity of 100 mM or less at pH of 1.5 to 3.9;

    2) adjusting the pH of the resulting sample from step (1) to pH of 4 to 8 to form particles, wherein the molarity of the adjusted sample is 100 mM or less; and

    3) removing the particles thereby to remove contaminant DNA in the sample.

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