Protein purification method
CAFCFirst Claim
Patent Images
1. A method for removing contaminant DNA in a sample containing a physiologically active protein, which comprises the following steps:
- 1) converting the sample containing a physiologically active protein into an acidic aqueous solution of low conductivity of 300 mS/m or less and having a molarity of 100 mM or less at pH of 1.5 to 3.9;
2) adjusting the pH of the resulting sample from step (1) to pH of 4 to 8 to form particles, wherein the molarity of the adjusted sample is 100 mM or less; and
3) removing the particles thereby to remove contaminant DNA in the sample.
1 Assignment
1 Petition
Accused Products
Abstract
A method for removing contaminant DNA in a sample containing a physiologically active protein, which comprises the following steps:
- 1) converting the sample containing a physiologically active protein into a neutral aqueous solution of low conductivity; and
- 2) removing the resulting particles.
37 Citations
13 Claims
-
1. A method for removing contaminant DNA in a sample containing a physiologically active protein, which comprises the following steps:
-
1) converting the sample containing a physiologically active protein into an acidic aqueous solution of low conductivity of 300 mS/m or less and having a molarity of 100 mM or less at pH of 1.5 to 3.9; 2) adjusting the pH of the resulting sample from step (1) to pH of 4 to 8 to form particles, wherein the molarity of the adjusted sample is 100 mM or less; and 3) removing the particles thereby to remove contaminant DNA in the sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
-
-
13. A method for removing contaminant DNA in a sample containing a physiologically active protein, which comprises:
-
1) converting the sample containing a physiologically active protein into an acidic aqueous solution of low conductivity of 300 mS/m or less and having a molarity of 100 mM or less at pH of 1.5 to 3.9; 2) neutralizing the pH of the resulting sample from step (1) by addition of a buffer to raise the pH to a neutral level to form particles, wherein the molarity of the neutralized sample is 100 mM or less; and 3) filtering the resulting sample from step (2) to remove particles containing contaminant DNA.
-
Specification