Modified G-CSF polypeptide

• US 8,058,398 B2
• Filed: 04/25/2005
• Issued: 11/15/2011
• Est. Priority Date: 01/28/1993
• Status: Expired due to Fees

• Current Assignee: Amgen Inc.
• Original Assignee: Amgen Inc.
• Inventors: Osslund, Timothy D.
• Primary Examiner(s): Chandra; Gyan
• Application Number:
  US11/113,581
• Publication Number:
  US 20050260154A1
• Time in Patent Office: 2,395 Days
• Field of Search: None
• US Class Current: 530/351
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61P 13/12 of the kidneys
• CPC Class Codes: A61P 17/02 for treating wounds, ulcers, burns, scars, keloids, or the like
• CPC Class Codes: A61P 19/00 Drugs for skeletal disorders
• CPC Class Codes: A61P 31/04 Antibacterial agents
• CPC Class Codes: A61P 31/10 Antimycotics
• CPC Class Codes: A61P 35/00 Antineoplastic agents
• CPC Class Codes: A61P 35/02 specific for leukemia
• CPC Class Codes: A61P 37/04 Immunostimulants
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: A61P 7/00 Drugs for disorders of the blood or the extracellular fluid
• CPC Class Codes: A61P 7/06 Antianaemics
• CPC Class Codes: A61P 9/00 Drugs for disorders of the cardiovascular system
• CPC Class Codes: C07K 1/00 General methods for the preparation of peptides , i.e. processes for the organic chemical preparation of peptides or proteins of any length
• CPC Class Codes: C07K 14/535 Granulocyte CSF; Granulocyte-macrophage CSF
• CPC Class Codes: C07K 2299/00 Coordinates from 3D structures of peptides, e.g. proteins or enzymes
• CPC Class Codes: C07K 2319/00 Fusion polypeptide
• CPC Class Codes: Y02A 90/10 Information and communication technologies [ICT] supporting adaptation to climate change, e.g. for weather forecasting or climate simulation

Abstract

The present invention relates to granulocyte colony-stimulating factor (“G-CSF”) hybrid molecules which retain the internal core helices of G-CSF. Also provided are pharmaceutical compositions containing hybrid molecules.

Claims

1. A modified G-CSF polypeptide comprising the amino acid sequence shown in SEQ ID NO:

2 and

a first chemical moiety attached directly to an external loop;

a second chemical moiety attached to the first chemical moiety;

wherein the first chemical moiety is a polysaccharide and the second chemical moiety is polyethelene glycol;

wherein the modified G-CSF polypeptide has ability to selectively stimulate the production of neutrophils; and

wherein the N terminal methionine as set forth in SEQ ID NO;

2 is optional.

2. The modified G-CSF polypeptide of claim 1 wherein the external loop is CD loop.

3. The modified G-CSF polypeptide of claim 1 wherein the external loop is AB loop.

4. The modified G-CSF polypeptide of claim 1 wherein the external loop is BC loop.